Chang Gung University

Kung-Yuan Chang

Min-Hsien Wu

Tung-Ming Pan

Shiao-Wen Tsai

Chao-Wen Lin

A detection method for a sensor membrane formed of europium titanium oxide as part of a biosensor by using PNIPAAm for wrapping enzymes includes adding 1.0 g of NIPAAm powder to 20 ml water, heating same at 60&#xb0; C. to form NIPAAm solution, and cooling the NIPAAm solution; adding 200 &#x3bc;l of 98.7 wt % of APS and 50 &#x3bc;l of 99 wt % of TEMED to the NIPAAm solution, uniformly mixing same, and reacting the mixture for 30 hours to prepare a transparent, gel PNIPAAm; adding 5 mg enzymes to 100 &#x3bc;l of 1&#xd7;PBS buffer solution, uniformly mixing same, adding 100 &#x3bc;l of PNIPAAm to the buffer solution, and uniformly mixing the buffer solution; placing a biosensor on a heater for heating at a constant temperature of 37&#xb0; C. with the biosensor being an EIS sensor having a sensor membrane formed of EuTiO; and taking a measurement.

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Caotun, Taiwan

Kung-Yuan Chang