Danisco US Inc.

Kun Zhong

Jing Ge

Zhongmei Tang

Jayarama K Shetty

Bo Zhang

Karsten Matthias Kragh

Sung Ho Lee

Shukun Yu

Ling Hua

Xiaogang Gu

Zhengzheng Zou

Helong Hao

Jalsen Li

Jinahua (Jalsen) Li

Wenting Li

Bo Zhong

Described are methods for saccharifying starch-containing materials using a glucoamylase, methods for producing fermentation products, and fermentation products produced by the method thereof as well as methods for increasing starch digestibility in a ruminant using at least one of the glucoamylases described herein.

Glucoamylases and methods of use, thereof

Fungal glucoamylases from&#x2014;expressed inhost cells (AfGATR) are provided.host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such asalpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed byglucoamylase (AnGA) or native AfGA expressed in. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.

host cells expressing a glucoamylase fromand methods of use thereof

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Shanghai, China

Kun Zhong