Peoplebio, Inc.

Kun Taek Lim

Hyun Jung Oh

Seong Soo Alexander An

Shin Won Kim

Kwan Soo Lee

Gwang Je Kim

Byoung Sub Lee

Ji Sun Yu

The present invention relates to a method for detecting an aggregate of an aggregate-forming polypeptide of a biosample, comprising (a) a step of spiking a dimer type of the aggregate-forming polypeptide into the biosample to be analyzed; (b) a step of incubating a resultant product of step (a) to further form an aggregate of the aggregate-forming polypeptide; (c) a step of contacting a resultant product of step (b) with a binding agent-tag in which a signal-generating tag is bound to a binding agent that binds to the aggregate of the aggregate-forming polypeptide; and (d) a step of detecting a signal which is generated from the binding agent-tag bound to the aggregate of the aggregate-forming polypeptide.

Method for detecting aggregate of aggregate-forming polypeptide

A method for differentially detecting a multimeric form from a monomeric form of a multimer-forming polypeptide in a biosample includes (a) contacting the biosample to a capturing antibody recognizing an epitope on the multimer-forming polypeptide to capture the monomeric form, multimeric form or monomeric and multimeric forms; (b) contacting the monomeric form, multimeric form or monomeric and multimeric forms captured to a detecting antibody recognizing an epitope identical to or overlapped with the epitope of step (a); and (c) detecting the formation of a multimeric form-detection antibody complex.

Differential detection of multimeric and monomeric forms of multimer-forming polypeptides

The present invention relates to a method for differentially detecting a multimeric form from a monomeric form of a multimer-forming polypeptide in a biosample, which comprises the steps of: (a) preparing a carrier-capturing antibody conjugate by binding a capturing antibody to the surface of a solid phase carrier in a three dimensional manner, wherein the capturing antibody is capable of recognizing an epitope on the multimer-forming polypeptide; (b) preparing a detection antibody, wherein an epitope recognized by the detection antibody is present at a position in the multimer-forming polypeptide to cause a steric hindrance by the capturing antibody bound to its epitope to prevent the binding of the detection antibody to the multimer-forming polypeptide; (c) contacting simultaneously the carrier-capturing antibody conjugate and the detection antibody to the biosample; and (d) detecting the formation of a carrier-capturing antibody-multimeric form-detection antibody complex.

Methods for differentially detecting a multimeric form from a monomeric form of a multimer-forming polypeptide through three-dimensional interactions

Growing community of inventors

Seoul, South Korea

Kun Taek Lim