Genefrontier Corporation

The University of Tokyo

Chiba University

Kumiko Tsuihiji

Kanehisa Kojoh

Takashi Kanamori

Shizue Katoh

Mikiko Nakamura

Yuki Hayami

Takuya Ueda

Tomoe Fuse

Shizue Kato

Toshinori Nakayama

Akira Miyakoshi

Chiaki Iwamura

The present invention provides an antibody that specifically binds to human CD69, has an activity to suppress allergic inflammation, and has cross-reactivity with mouse CD69. In addition, the present invention provides an antibody having high binding affinity for human CD69 and an activity to suppress allergic inflammations. The antibody of the present invention can be a human antibody.

Anti-human CD69 antibody, and use thereof for medical purposes

The invention provides a method of producing a protein multimer-nucleic acid complex comprising a protein multimer and any one target component of the protein multimer. A nucleic acid encoding the target component is subjected to in vitro translation to provide a translation product containing a target component-nucleic acid complex of the target component and the nucleic acid encoding the target component. A nucleic acid encoding a non-target component constituting the protein multimer together with the target component is translated into the non-target component by adding the nucleic acid encoding the non-target component to the previously provided translation product to provide the non-target component. The non-target component then is associated with the target component contained in the target component-nucleic acid complex to form a protein multimer, thus affording the protein multimer-nucleic acid complex of the protein multimer and the nucleic acid encoding the target component.

Efficient method for displaying protein multimer

According to the present invention, a composition possessing cell-free protein synthesis activity with reduced contaminating lipopolysaccharide, and a method for producing a protein using the same are provided. When ribosome display is performed using the composition and method for protein production of the present invention, the background that is caused by non-specific binding is reduced, so that a nucleic acid that encodes the desired polypeptide can be selected with high accuracy and high efficiency.

Composition for synthesizing protein with reduced lipopolysaccharide contamination, method for producing protein using said composition

Growing community of inventors

Kashiwa, Japan

Kumiko Tsuihiji