Tohoku University

Japan Aviation Electronics Industry, Limited

Seikagaku Corporation

Kumi Inoue

Tomokazu Matsue

Atsushi Suda

Ryota Kunikata

Kosuke Ino

Hiroyuki Hayashi

Hitoshi Shiku

Takahiro Ito

Yusuke Kanno

Toshio Oda

Tatsuo Kimura

Hikaru Mizumura

Miho Ikegawa

Kentaro Ito

An electrochemical measurement apparatus includes: a tank containing electrolytic solution and a sample that generates or consumes measurement target substances in the electrolytic solution; a plurality of uniformly mixed working electrodes; and a counter electrode; the apparatus adapted to simultaneously apply a voltage between each of the working electrodes and the counter electrode; and the apparatus configured to measure a current that flows between each of the working electrodes and the counter electrode; wherein any two working electrode groups are mutually different in at least any of the determined voltage, presence/absence of a molecular modification of an electrode surface, and a species of the molecular modification; and whereby a distribution in measurement area of the currents that flow through the working electrodes is acquired.

Electrochemical imaging method

Electrochemical measurement apparatus and transducer

An electrochemical measurement method is provided in which a working electrode that causes an oxidation-reduction reaction with a measurement target and a counter electrode connected to the working electrode are provided in an electrolytic solution containing the measurement target, and a measuring voltage is applied between the working electrode and the counter electrode to measure a current that flows between the working electrode and the counter electrode in proportion to the amount of the measurement target, wherein an eliminating electrode is provided in the electrolytic solution, and the method performs: eliminating the measurement target by applying an eliminating voltage, which has the same polarity as the measuring voltage, between the eliminating electrode and the counter electrode to oxidize or reduce the measurement target; diffusing a new measurement target; and measuring the current by applying the measuring voltage between the working electrode and the counter electrode.

Electrochemical measurement method, electrochemical measurement device and transducer

An electrochemical measurement device for measuring a chemical substance generated or consumed in a biological sample in a solution includes electrode surfaces, a spacer, and at least one wall plate. The electrode surfaces, the spacer, and the wall plate are arranged on the same flat surface. Each of the electrode surfaces has a diameter dnot more than 80 μm. A height of the spacer has a predetermined value within a range given by h=21.8(d+0.8)/(d+9.7)±5 [μm]. The spacer has a structure in which an enclosed three-dimensional region is not formed by the biological sample, the flat surface, and the spacer while the biological sample is in contact with the spacer. The wall plate has a property of being impervious to a dissolved substance in the solution and has a height not less than the height of the spacer. Two of the electrode surfaces are separated by the wall plate.

Electrochemical measurement device and transducer

Provided are a device and a method for rapidly and simply detecting endotoxin without using an expensive reagent. The endotoxin detection device includes: a region containing an electrolyte solution; a partitioning member that partitions the region into two compartments such that the two compartments are in communication via a nanopore; a first electrode that is disposed in a first compartment; a second electrode that is disposed in a second compartment and is electrically connected to the first electrode; an electrolyte solution flow generating means that causes electrolyte solution in the first compartment to move to the second compartment via the nanopore; an application means that applies voltage between the first electrode and the second electrode; and a monitoring means that monitors current.

Endotoxin detection device and endotoxin detection method

An electrochemical measurement device for measuring a chemical substance generated or consumed in a biological sample in a solution includes electrode surfaces, a spacer, and at least one wall plate. The electrode surfaces, the spacer, and the wall plate are arranged on the same flat surface. Each of the electrode surfaces has a diameter dnot more than 80 &#x3bc;m. A height of the spacer has a predetermined value within a range given by h=21.8(+0.8)/(d+9.7)&#xb1;5 [&#x3bc;m]. The spacer has a structure in which an enclosed three-dimensional region is not formed by the biological sample, the flat surface, and the spacer while the biological sample is in contact with the spacer. The wall plate has a property of being impervious to a dissolved substance in the solution and has a height not less than the height of the spacer. Two of the electrode surfaces are separated by the wall plate.

The present invention addresses the problem of providing a means for making highly sensitive and stable measurements possible using electrochemical measurement methods. This problem is resolved by providing: a labeling substance represented by general formula (1) and used to label a substrate; a measurement substrate that is formed by being labeled using the labeling substance; a method of measuring using electrochemical measurement methods that use the measurement substrate; and a reagent kit that includes as components the labeling substance and/or the measurement substrate. (In general formula (1), Ris either H or an alkyl group (CH), m is an integer of 1-4, Ris an alkyl group (CH), and n is an integer of 1-4.)

Electrochemical measurement using phenylenediamine derivative

An electrochemical measurement method is provided in which a working electrode and a counter electrode are provided in an electrolytic solution containing the measurement target and a measuring voltage is applied between the working electrode and the counter electrode to measure a current flowing between the working electrode and the counter electrode in proportion to the amount of the measurement target. A measurement target eliminating thin-wire electrode made of thin wire stretched in such a shape that extends from one point on the bottom into space in the electrolytic solution well and back to another point on the bottom is provided. The electrochemical measurement method includes: eliminating the measurement target by applying an eliminating voltage having the same polarity as the measuring voltage between the measurement target eliminating thin-wire electrode and the counter electrode; and diffusing a new measurement target after stopping the application of the eliminating voltage.

Electrochemical measurement method, electrochemical measurement apparatus and transducer

An electrochemical measurement method for electrochemically measuring a chemical substance generated or consumed in a biological sample in a solution is provided which includes performing measurement by placing the biological sample at a distance away from an electrode surface in the direction perpendicular to the electrode surface. The distance is determined in advance on the basis of simulation in which a current flows through a working electrode.

An electrode chip including one auxiliary cell and a plurality of measurement cells on a substrate, where the auxiliary cell includes a reference electrode, a counter electrode, and an auxiliary cell-side working electrode, the measurement cell includes a driving electrode and a measurement cell-side working electrode, and the auxiliary cell-side working electrode and the measurement cell-side working electrode are electrically connected.

Electrode chip

An electrode device for an electrochemical sensor chip includes an insulation sheet having an insulating property and including a top surface and a bottom surface opposite to each other in a thickness direction, and electrode members having a conductivity and held by the insulation sheet with the electrode members piercing the insulation sheet in a thickness direction, one ends of the electrode members located on the top surface side of the insulation sheet being connected to an analyte, the other ends located on the bottom surface side of the insulation sheet being connected to an electrodes of a transducer, at least the one ends of the electrode members being made of a mixture of conductive particles and an insulating material.

Growing community of inventors

Miyagi, Japan

Kumi Inoue