Insilixa, Inc.

Kshama Jirage

Arjang Hassibi

Arun Manickam

Yang Liu

Kaveh Milaninia

Rituraj Singh

Andrea Cuppoletti

Lei Pei

The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.

Methods and compositions for surface functionalization of optical semiconductor-integrated biochips

The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.

Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays

The present disclosure provides methods, devices and systems that enable simultaneous multiplexing amplification reaction and real-time detection in a single reaction chamber.

Methods and systems for multiplex quantitative nucleic acid amplification

Growing community of inventors

Palo Alto, CA, United States of America

Kshama Jirage