University of Utah Research Foundation

Illumina Corporation

Illumina Cambridge Limited

Illumina Singapore Pte. Ltd.

Krystal Sly

Hai Quang Tran

Alexandre Richez

Lewis J Kraft

Sean M Ramirez

Brian Douglas Mather

Xavier Von Hatten

Ludovic Vincent

Hong Xie

Julia Morrison

Gianluca Andrea Artioli

Wei Wei

Edwin Li

Maxwell Zimmerley

Hayden Black

Innsu Daniel Kim

Ryan Sanford

John Conboy

Sojeong Moon

An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

Protective surface coatings for flow cells

Disclosed are methods of detecting an analyte of interest comprising introducing a sample comprising an analyte of interest to an antibody or antibody fragment; incubating the sample and antibody or antibody fragment under conditions sufficient to allow binding of the analyte of interest to the antibody or antibody fragment; and detecting the binding of the analyte of interest to the antibody or antibody fragment using a label-free second harmonic detection system. Also disclosed are methods of screening and diagnosing using antibodies or antibody fragments and a label-free second harmonic detection system.

Systems and methods for label-free detection of analytes

Growing community of inventors

San Diego, CA, United States of America

Krystal Sly