E.i. Dupont De Nemours and Company

Kristy N Kostichka

Qiong Cheng

Michael G Bramucci

Vasantha Nagarajan

Stuart M Thomas

Hong Peng Wang

Pierre E Rouviere

Hongxian He

Stephen R Fahnestock

Jean-Francois Tomb

Patricia C Brzostowicz

Luan Tao

Mario W Chen

Katharine Janet Gibson

Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.

Peptide linkers for effective multivalent peptide binding

A plasmid has been isolated fromstrain AN12 comprising a unique replication protein. The replication protein may be used in a variety of cloning and expression vectors and particularly in shuttle vectors for the expression of heterologous genes insp.

[object Object]

Genes have been isolated from a variety of bacteria encoding Baeyer-Villiger monooxygenase activity. The genes and their products are useful for the conversion of ketones to the corresponding esters. A series of motifs, common to all genes, has been identified as diagnostic for genes encoding proteins of this activity.

Genes encoding Baeyer-Villiger monooxygenases

Genes have been isolated fromAN12 strain encoding the isoprenoid biosynthetic pathway. The genes and gene products are the first isolated from astrain. The genes and gene products of the present invention may be used in a variety of ways for the production of isoprenoid compounds in a variety of organisms.

Genes involved in isoprenoid compound production

Rhodococcus cloning and expression vectors

A 10 kb gene cluster has been isolated from SC1 comprising genes encoding enzymes useful for the synthesis of dodecanoic diacid from cyclododecanone and other cyclic intermediates. The six specific open reading frames have been identified that are associated with dodecanoic diacid biosynthesis. In addition to the expected substrates the enzymes of the instant invention have moderate specificity for C11-C15 compounds.

Genes involved in cyclododecanone degradation pathway

Growing community of inventors

Wilmington, DE, United States of America

Kristy N Kostichka