Life Technologies Corporation

Ultima Genomics, Inc.

Kristopher Barbee

Gilad Almogy

Nathan Beckett

Steven Menchen

Denis Pristinski

Mark Pratt

Avishai Bartov

Dumitru Brinza

Abizar Lakdawalla

Maximilian Carpino

Derek Schulte

Jamie Michael Sullivan

Sean McCusker

Nicholas Peter Bajka

Ray Wheeling

Wolfgang Hinz

James M Bustillo

Shifeng Li

John F Davidson

Rui Zheng

Jonathan M Schultz

John Harris Leamon

Jon A Hoshizaki

Phillip You Fai Lee

Jacob A Wolf

Nathan Caswell

Ryan Jones

Jonathan O Schultz

Joseph Anthony

Jose Martin Sosa

Stephanie Kubecka

Gene Polovy

Osip Schwartz

Raymond Alan Wheeling

Sean Mccusker

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.

Methods and systems for analyte detection and analysis

Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

Methods, devices, and systems for analyte detection and analysis

The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. In a preferred embodiment, the present teachings provide apparatuses and methods for automated enrichment of templated beads produced by PCR.

Method and apparatus for automated sample preparation

Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.

Methods for biological sample processing and analysis

A rotor assembly includes a rotor plate to rotate around a first axis; a bucket rotatably attached to the rotor plate and to rotate around a second axis; and a stop plate to rotate around the first axis relative to the rotor plate between an open position and a closed position, when in the closed position, the stop plate is to engage the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly.

Rotor plate and bucket assembly and method for using same

A sample preparation apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm. In addition, the sample preparation apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles, as well as a centrifuge for loading such particles onto a sensor array. The sample preparation apparatus can further include receptacles for holding containers of reagent solutions.

Centrifuge with variable angle buckets and method for loading a device

Method and apparatus for automated sample manipulation

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San Francisco, CA, United States of America

Kristopher Barbee