Heliae Development, LLC

Kristine Sorensen

Laura Carney

Craig Erny

Methods of treating contamination, particularly fungal contamination, in cultures ofwith hydrogen peroxide and salt are described herein. The method comprises dosing the culture comprising a concentration of salt with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Methods for treating a culture of haematococcus pluvialis for contamination using salt and hydrogen peroxide

Methods of treating contamination, particularly fungal contamination, in cultures ofwith hydrogen peroxide are described herein. The method comprises detecting the contamination and then dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

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Methods of treating lysis in cultures ofwith hydrogen peroxide are described herein. The method comprises dosing the culture comprising with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Methods of treating contamination, particularly fungal contamination, in cultures ofwith salt are described herein. The method comprises dosing the culture comprising with a concentration of salt based on the stage of the cells in the culturing process to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Methods of treating lysis in cultures ofwith hydrogen peroxide are described herein. The method comprises detecting lysis in the culture and then dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Methods for preventing lysis in a culture of haematococcus pluvialis using hydrogen peroxide

Culture compositions comprisingand hydrogen peroxide are described herein. The compositions comprise a concentration of hydrogen peroxide based on the stage of the cells in the culturing process to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

Methods of treating contamination, particularly fungal contamination, in cultures ofwith hydrogen peroxide are described herein. The method comprises dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.

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Chandler, AZ, United States of America

Kristine Sorensen