Life & Brain Gmbh

Rheinische Friedrich-Wilhelms-Universität

Rheinische Friedrich-wilhelms-universitaet

Kristin Roy

Oliver Brüstle

Harald Neumann

Michael Peitz

A method for obtaining human microglial precursor cells, comprising: (a) providing a cell population comprising neural precursor cells, wherein the cell population is obtainable from embryoid bodies differentiated from human pluripotent stem cells; (b) differentiating the cell population comprising neural precursor cells into microglial precursor cells by culturing in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors; (c) expanding and enriching microglial precursor cells in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors and 10 to 150 ng/ml GM-CSF; and (d) isolating microglial precursor cells comprising CD45-positive cells.

Growing community of inventors

Bonn, Germany

Kristin Roy