Janssen Biotech, Inc.

Kristen Wiley

Shalom Goldberg

Vadim Dudkin

Michael J McCauley

Rebecca Wissner

Engineered antibodies useful for site-specific conjugation by a transglutaminase are described. Also described are methods of site-specific conjugation of the antibodies, the site-specifically conjugated antibodies, and pharmaceutical compositions and uses related to the site-specifically conjugated antibodies.

Site-specific conjugation of glycosylated monoclonal antibodies with transglutaminase

Provided herein are methods for site-specific conjugation of glycan intact antibodies by a transglutaminase. According to particular embodiments, the reaction conditions are maintained or reduced to a low-ionic strength condition, which allows for efficient and fast conjugation without the need for antibody deglycosylation. Also described are pharmaceutical compositions and uses related to the conjugation method.

Growing community of inventors

Trappe, PA, United States of America

Kristen Wiley