Lallemand Hungary Liquidity Management LLC

Stellenbosch University

Danstar Ferment Ag

Universiteit Stellenbosch

Kristen M Deleault

John E McBride

Emily Stonehouse

Allan C Froehlich

Mark Mellon

Riaan Den Haan

Haowen Xu

John S Bardsley

Elena E Brevnova

Aaron Argyros

Trisha Barrett

Nicky Caiazza

Charles F Rice

Erin Wiswall

Ryan Skinner

Anne K Warner

Chhayal V Gandhi

Shital A Tripathi

Kevin S Wenger

Frank Agbogbo

Indraneel Shikhare

Jennifer Gosselin

Yin-Ying Chiu

Abigail Foster

Alan Belcher

Vineet B Rajgarhia

Heidi H Hau

Merja Elisa Penttila

Vineet Rajgarhia

Matti Siika-Aho

Marja Ilmen

Alan Benjamin Gilbert

Sanni Voutilainen

Jaana Uusitalo

Anu Koivula

Deidre Willes

Lee R Lynd

Emile Van Zyl

Danie Lagrange

Mitchell Codd

Zachary Losordo

John Evan Eck McBride

Jim Flatt

Heidi Lau

Naomi Thorngren

John Mcbride

Emily A Stonehouse

Dan Murphy

Heidi Hanson Hau

Jeffrey D Keating

Jeff Villari

Elena Brevnova

Chhayal Ghandi

Alan Froehlich

Shaunita Rose

Merja Penttila

The present disclosure concerns recombinant microbial host cell having, in glycolytic conditions, increased glycerol importing activity glycerol as well as, in high osmotic conditions, a decreased NAD-dependent glycerol-3-phosphate dehydrogenase (GPD) activity. The recombinant microbial host cell is particularly useful for the fermentation of sugarcane- or molasses-based medium for the production of ethanol.

Optimization of biomass-based fermentations

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention is directed to the construction of prototrophic, cellulo lytic strains ofwith tethered and secreted cellulases and selection-based improvement of growth on cellulose by these strains. In some embodiments, host cells of the invention are able to produce ethanol using crystalline cellulose as a sole carbon source.

Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases

The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability ofto produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

Host cells, comprisingexpressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.

Growing community of inventors

Canaan, NH, United States of America

Kristen M Deleault