Monsanto Technology LLC

Greenlight Biosciences, Inc.

United States of America, As Represented by the Secretary of Agriculture

Krishnakumar Sridharan

Stanislaw Flasinski

Catherine Alice Chay

David Joseph Bowen

Arlene R Howe

Todd A Ciche

Xudong Ye

Elysia K Krieger

Ervin D Nagy

Thais Barros Rodrigues

Suresh D Desai

Wimalanathan Kokulapalan

Anna N Underhill

Yufeng Fang

Lance E Cadle Davidson

Upendra Kumar Devisetty

Sambit Kumar Mishra

Adriana Puralewski

Christopher Lawrence

A pesticidal protein class exhibiting toxic activity against Coleopteran and Lepidopteran pest species is disclosed, and includes, but is not limited to, TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Coleopteran and Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Coleopteran and Lepidopteran species pests using the TIC7040, TIC7042, TIC7381, TIC7382, TIC7383, TIC7386, TIC7388, and TIC7389 pesticidal proteins are also provided.

Insect inhibitory proteins

This disclosure is related to plant-optimized recombinant nucleic acids encoding Cpf1 and their use in planta. Also disclosed are compositions, expression cassettes, and plant cells comprising the recombinant nucleic acids as well as methods and kits for modifying a target sequence in a plant genome using the recombinant nucleic acids.

Compositions and methods for genome editing in planta

Disclosed herein are compositions and methods that involve recombinant polynucleotide molecules, such as single or double-stranded DNA or RNA molecules, also referred to 'triggers', that are useful for controlling or preventinginfection, or recombinant DNA constructs for making such RNA molecules or for making transgenic grape plants resistant toinfection. In some embodiments, polynucleotide triggers are provided as topically applied agents for controlling or preventing infection of a plant by

RNA-based control of powdery mildew

Provided herein are methods for using RNAi molecules targeting a proteasome beta 5 (PSMB5) gene for controlling Coleopteran insects, methods for producing RNAi molecules targeting PSMB5, and compositions comprising RNAi molecules targeting PSMB5.

Control of insect infestation

Provided herein are methods for using RNAi molecules targeting an Inhibitor of Apoptosis (IAP) gene for controlling Coleopteran insects, methods for producing RNAi molecules targeting IAP, and compositions comprising RNAi molecules targeting IAP.

Control ofinsects

Growing community of inventors

Cary, NC, United States of America

Krishnakumar Sridharan