Atherotech, Inc.

The Uab Research Foundation

Ningbo Alabama, LLC

Other

Vap Diagnostics Laboratory, Inc

Krishnaji R Kulkarni

David W Garber

Jere P Segrest

Larry M Glasscock

Alice Glasscock

The present disclosure provides a variety of methods based on the determination of cholesterol concentration in remnant lipoprotein and/or the determination of a remnant lipoprotein level in a sample from a subject. Such methods include, but are not limited to, a method of predicting the risk of cardiovascular disease, a method for evaluating a subject for treatment for a cardiovascular disease and a method for determining if a subject is suffering from cardiovascular disease.

Use of remnant lipoprotein in the treatment and prevention of cardiovascular disease

Although a more accurate estimate of a person's risk of cardiovascular disease can be made on the basis of the number of lipoprotein particles per unit volume in the person's blood, current methods all rely on measuring the mass of lipoprotein cholesterol per unit volume. It has been discovered that a rapid and accurate lipoprotein particle count can be obtained by photometry. A method and apparatus are provided for measuring the number of lipoprotein particles in a sample using photometry.

Measurement of serum lipoproteins

The present disclosure provides methods to predict the risk of CHD and/or clinical manifestations of CHD in a subject. In one embodiment, the method involves measuring the levels or concentration of apo A1, a subclass of HDL, HDL, or a combination of the foregoing. The methods of the present disclosure are particularly useful when the subject has reached target levels of one or more lipoproteins, such as, but not limited to, LDL or HDL or subclass of the foregoing.

Methods for determining the risk of coronary heart disease and clinical manifestations of coronary heart disease

A system and method for measuring apolipoprotein B(apo B) during the cholesterol subclass measurement process is provided which obviates the need for a separate apo B test.

Apo B measurement system and method

continuous flow analysis system measures the cholesterol distribution among different lipoprotein classes in a blood sample. A blood plasma sample is separated into different lipoprotein classes by single vertical spin density gradient ultracentrifugation. The sample is then introduced in a continuous succession into a reagent stream which flows continuously through a detector. A sensor detects the end of the sample in a sample stream before it is introduced into said reagent stream. A wash solution is introduced into the sample stream in response to detection of the end of said sample. The wash solution back flows through the sample stream into the sample container to wash any residue and/or air bubbles from the sample stream. After a predetermined time period, the sample stream is closed and the process is repeated for the next sample.

Controlled dispersion flow analysis system

A continuous flow analysis system measures the cholesterol distribution among different lipoprotein classes in a blood sample. A blood plasma sample is separated into different lipoprotein classes by single vertical spin density gradient ultracentrifugation. The sample is then introduced in a continuous succession into a continuous reagent stream which flows through a detector. A signal of the reaction, such as the absorbance of the reaction product, is continuously monitored as the reaction mixture flows through the detector. The detector measures a transient signal thus allowing a relatively short reaction period. The absorbance data is digitized and analyzed with the help of a computer to generate a cholesterol profile indicative of the cholesterol concentration in each lipoprotein class.

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Vestavia, AL, United States of America

Krishnaji R Kulkarni