Codex Dna, Inc.

Telesis Bio Inc.

Krishna Kannan

Daniel Glenn Gibson

J Craig Venter

Hamilton O Smith

Clyde A Hutchison

Ray-Yuan Chuang

Vladimir N Noskov

Bogumil J Karas

Chuck Merryman

Lin Ding

Kim S Wise

John I Glass

John E Gill

Lixia Fu

Methods for generating synthetic genomes, for example synthetic genomes having desired properties or viable genomes of reduced size, are disclosed. Also disclosed are synthetic genomes produced by the methods disclosed herein and synthetic cells containing the synthetic genomes disclosed herein.

Generation of synthetic genomes

The invention provides methods of synthesizing a product DNA molecule having a desired and/or defined sequence. The methods involve annealing at least one long oligonucleotide and at least one short oligonucleotide to at least one anchor strand having a sequence at least partially complementary to the at least one long and at least one short oligonucleotide. The invention also provides methods of synthesizing DNA molecules by assembling oligonucleotide members of a library that contains less than 20,000 members that can be assembled into all possible DNA sequences.

Demand synthesis of polynucleotide sequences

Growing community of inventors

San Diego, CA, United States of America

Krishna Kannan