Promega Corporation

Kris Zimmerman

Keith V Wood

Lance P Encell

Monika G Wood

Gediminas Vidugiris

Paul Otto

Rachel Friedman Ohana

Michael R Slater

Aldis Darzins

Kate Qin Zhao

Brock Binkowski

Thomas Machleidt

Andrew S Dixon

Marie Schwinn

Pete Stecha

Aldis Darzin

Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.

Internal protein tags

The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.

Mutant hydrolase proteins with enhanced kinetics and functional expression

A modified luciferase protein which is a sensor for molecules including cAMP is provided. The modified luciferase protein includes one or more heterologous amino acid sequences, at least one of which directly or indirectly interacts with cAMP.

Luciferase biosensors for cAMP

Mutant dehalogenase proteins

Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression

Growing community of inventors

Madison, WI, United States of America

Kris Zimmerman