Kumiai Chemical Industry Co., Ltd.

National Institute of Agrobiological Sciences

Kozo Nagayama

Tsutomu Shimizu

Koichiro Kaku

Kiyoshi Kawai

Atsunori Fukuda

Yoshiyuki Tanaka

Hiroyuki Yano

Yuji Misumi

Ishizue Nakayama

Jun Toyoshima

Koji Yamaji

Katsushi Toyooka

Disclosed is a gene coding for a protein (a) or (b) showing a high level of resistance to PC herbicides or sulfonylurea herbicides. The protein (a) consists of an amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8, and the protein (b) consists of an amino acid sequence derived from the amino acid sequence of any one of SEQ ID NOS: 2, 4, 6 and 8 by substitution, deletion or addition of at least one or more amino acids, has resistance to a pyrimidinyl carboxy herbicide, and has acetolactate synthase activity.

Gene coding for acetolactate synthase

The present invention provides a method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor, comprising the steps of: (a) and (b), which (a) identifies an amino acid in an amino acid sequence of a scytalone dehydratase isolated from the rice blast fungus, where the amino acid corresponds to valine at position 75 of SEQ ID NO: 4; and (b) assesses the sensitivity of the rice blast fungus to the scytalone dehydratase inhibitor, where if the amino acid identified in step (a) is methionine, the sensitivity of the rice blast fungus to The scytalone dehydratase inhibitor is assessed to be lower than that of a wild-type rice blast fungus which endogenously produces the scytalone dehydratase of SEQ ID NO: 4.

Method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor

The present invention provides a gene that can be used extensively in studies relating to resistant rice blast fungi. The gene codes for either one of the following proteins (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO:2; or (b) a protein consisting of an amino acid sequence shown in SEQ ID NO:2 by deletion, substitution or addition of one or more amino acids, which exhibits scytalone dehydratase activity in the presence of a scytalone dehydratase inhibitor.

Scytalone dehydrogenase gene showing tolerance to agricultural pesticide

The present invention provides a gene encoding ALS protein which shows a extremely high level of resistance to pyrimidinyl carboxy herbicides. The gene of the present invention encodes an acetolactate synthase protein, for instance, according to SEQ ID NO:1, where the acetolactate synthase protein has resistance to a pyrimidinyl carboxy herbicide and has acetolactate synthase activity.

Gene encoding acetolactate synthase

The present invention relates to a novel strain ofsp. D747 (deposited as FERM BP-8234) and methods for controlling plant diseases and insect pests, comprising administering cultures ofsp. D747 (including the viable bacteria) or viable bacteria isolated by culturing, on the plant parts such as roots, stems, leaves, seeds, and the like, or in the culture soil.

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The present invention provides a wettable composition for use in agriculture comprising microorganisms as an active ingredient, which can maintain a high survival rate of the same over long periods, and exhibit superior physical properties during applications, and provide a method for stably storing the wettable composition for use in agriculture for a long period of time. That is, the present invention corresponds to a wettable composition for use in agriculture comprising spores of filamentous fungi which are in the form of dried powders having a volume median diameter ranging from 2 &mgr;m to 10 &mgr;m, and having a ratio of (volume 90% diameter)/(volume 10% diameter;) of not more than 5.62, and an adsorbent having a water absorption ability in an amount of at least 1% by mass based on the total mass of the composition. The composition is prepared by pulverizing and mixing a mixture of spores of filamentous fungi, an adsorbent having a water absorption ability, and other additive components such as a surfactant, in an appropriate ratio of the same, by means of impact pulverization, and adjusting a moisture content so that the moisture content is less than 2.5% by mass based on the total mass of the composition. In addition, the composition is stored by packaging in a bag formed from a hermetic film.

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Shizuoka, Japan

Kozo Nagayama