Ensuiko Sugar Refining Co., Ltd.

Other

Bio Research Corporation of Yokohama

Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo

Kaken Pharmaceutical Company, Ltd.

National Institute of Advanced Industrial Science and Technology

Amano Enzyme Inc.

Society for Techno-innovation of Agriculture, Forestry and Fisheries

Ensuiko Sugar Regining Co., Ltd.

Ensuiko Seito Kabushiki Kaisha

Tadakatsu Mandai

Director of National Food Research Institute, Minstry of Agriculture,

Kozo Hara

Katsuhiko Mikuni

Koji Hara

Hiroshi Okumoto

Koki Fujita

Hitoshi Hashimoto

Katsuyoshi Nakanishi

Toshio Miyake

Tetsuya Ito

Shuzo Sakai

Sumio Kitahata

Masayuki Yamashita

Hiroki Hamada

Teruhiko Umetsu

Kohki Fujita

Yasuhiko Tsunetomi

Hiroyuki Hashimoto

Masamichi Okada

Nobuhiro Kuwahara

Shigeharu Mori

Hideki Takahashi

Shoichi Kobayashi

Kyoko Koizumi

Hirofumi Nakano

Isamu Uemasu

Toshiya Takano

Mitsuru Monma

Toshiko Tanimoto

Yoshinori Tsuchiya

Kenichi Hamayasu

Teruo Nagano

Takuzo Shibata

Yoshiyuki Sakano

Ikuo Yashiki

Takashi Tonozuka

Kosho Nakamura

A novel method of very efficiently elevating the yield of oligosacchrides containing &#x3b1;-galactosyl, compared with the conventional methods, which comprises treating galactose or a galactose-containing material with a specific &#x3b1;-galactosidase and thus performing a dehydrocondensation reaction at a high substrate concentration. Anti-candida compositions originating in foods, having a high safety and excellent anti-candida effect and not being restricted in the supply, which contain as the active ingredient oligosacchrides obtained by treating galactose or a galactose-containing material with an &#x3b1;-galactosidase and thus performing a dehydrocondensation.

Method of elevating yield of oligosaccharides containing &#x3b1;-galactosyl and anti-candida compositions

In a reaction system having at least two liquid-liquid interfaces between an organic phase of raw material containing a compound(s) to be separated and an aqueous phase of an aqueous solution of inclusion-complexing agent and between that aqueous phase and an organic phase(s) of extraction solvent(s), the compound(s) to be separated is entrapped into the aqueous phase through formation of an inclusion complex(es) of the inclusion-complexing agent with the compound(s), while the compound(s) is entrapped into the organic phase(s) of extraction solvent(s) through dissociation of said inclusion complex(es). The foregoing operation is performed using, for example, a squarish U-shaped tube or an H-shaped tube with bottom plates. Preferred examples of inclusion-complexing agent include highly water-soluble branched cyclodextrins.

Method and equipment for continuous and selective inclusion separation

The object of the invention is to develop baccatin derivatives useful for preparing taxoid compounds such as paclitaxel and a process for the producing the same. The invention provides baccatin derivatives represented by the general formula (I) as well as a process for producing baccatin derivatives represented by the general formula (I) above, which comprises allowing a baccatin represented by the general formula (II) to react with a &bgr;-ketoester in the absence of a catalyst or in the presence of a tin compound or an amine base, preferably under reduced pressure.

Baccatin derivatives and a process for producing the same

Taxoid derivatives and process for producing the same

A &bgr;-fructofuranosidase gene coding for a protein having an amino acid sequence described in Seq. I.D. No. 1 in the Sequence Listing. The gene enables production of &bgr;-fructofuranosidase on a large scale more easily and less dependently on the productivity of microorganisms than the production by cultivation of the microorganisms. Also, the &bgr;-fructofuranosidase gene is useful for the development of variant enzymes that have increased heat resistance and transfer ratio by means of genetic engineering techniques.

&bgr;-fructofuranosidase gene

A taxoid compound represented by general formula (I) ##STR1## where the symbols R.sup.1, R.sup.2, R.sup.3, R.sup.4, Bz, and Ac have specified meanings. Also, a method for producing the taxoid derivative from a baccatin derivative having a .beta.-ketoester group at the 13-position by transesterification and a method for producing taxoid compound such as paclitaxel from the taxoid derivative under mild condition are disclosed.

Taxoid compound and method for producing the same

Disclosed are ursodeoxycholic acid derivatives having an increased solubility in water, and methods for producing the derivatives. To produce the derivatives, ursodeoxycholic acid is protected at its carboxyl group with a benzyl group, then reacted with tetrabenzyl-acetic acid-oxyglucoside of the following formula (1), and de-benzylated; or ursodeoxycholic acid is protected at its hydroxyl group with a t-butyldimethylsilyl group, then reacted with tetrabenzyl-2-hydroxyethyloxyglucoside of the following formula (2), and de-t-butyldimethylsilylated and de-benzylated. ##STR1##

Ursodeoxycholic acid derivatives and methods for producing them

A sugar derivative wherein a hydroxy-carboxylic acid is combined to a position 1 of a sugar via ether linkage, and other hydroxyl groups are protected with a protective groups. A method of producing the sugar derivative, comprises allowing glycolic ester to react with a compound so as to combine said glycolic ester at the position 1 of the sugar skeleton, and then saponifying, said compound has on the position 1 any of hydroxyl group, halogen atom and sulfur atom with a substitutent group, and has other hydroxyl groups being protected with a protective groups.

Process for acylating the 1-position of saccharides

A taxoid derivative wherein sugar is combined with any one of paclitaxel, docetaxel and 10-deacetyl-bacatin III via a spacer. A method of producing the taxoid derivative comprises protecting hydroxyl groups at specific position of paclitaxel or docetaxel by protective compound followed by reacting with tetrabenzyl acetyloxyglucoside, and then carrying out debenzyl and detriethylsilyl reactions. A method of producing the taxoid derivative comprises reacting paclitaxel or docetaxel with tetrabenzyl acetyloxyglucoside, and then carrying out debenzyl reaction.

Taxoid derivative and method of producing thereof

Disclosed are novel branched cyclodextrins having any of an N-acetylglucosamine group, a glucosaminyl group, an N-acetylgalactosaminyl group and a galactosaminyl group as bonded to the hydroxyl group of the cyclodextrin ring by .beta.-bonding therebetween, and their salts. The novel branched cyclodextrins are produced by reacting a solution containing a cyclodextrin and an N-acetylglucosamine or N-acetylgalactosamine with an N-acetylhexosaminidase, and optionally deacetylating the resulting product.

Branched cyclodextrins and method for producing them

The lactosucrose high-content powder according to the present invention is prepared by allowing an aqueous solution containing sucrose and lactose to act on a saccharidetransferring enzyme, removing concomitant saccharides in the resultant saccharide solution containing lactosucrose to obtain a solution having a high concentration of lactosucrose, i.e. lactosucrose content of 45 w/w % or more of the sugar composition, and spraydrying the resultant solution to obtain a powder having a high concentration of lactosucrose. The powder is incorporated in an orally- or parenterally-administrable product to obtain an orally- or parenterally-administrable product which exerts a selective growth-promoting-effect on a microorganism of the genus Bifidobacterium in vivo.

Process for preparing a powder having a high concentration of

The lactosucrose high-content powder according to the present invention is prepared by allowing an aqueous solution containing sucrose and lactose to act on a saccharide-transferring enzyme, removing concomitant saccharides in the resultant saccharide solution containing lactosucrose to obtain a lactosucrose high-content solution with a lactosucrose content of 45 w/w % or higher on sugar composition, and spray-drying the resultant solution to obtain a lactosucrose high-content powder. The powder is incorporated in an orally- or parenterally-administrable product to obtain an orally- or parenterally-administrable product which exerts a selective growth-promoting-effect on a microorganism of the genus Bifidobacterium in vivo.

Process for preparing lactosucrose high-content power

An enzymatic method for the preparation of a fructose-containing oligosaccharide, in which a .beta.-fructofuranosidase obtained by culturing Arthrobacter sp. K-1 (FERM BP-3192) as an enzyme is reacted on sucrose, raffinose or stachyose as the donor in the presence of an aldose or ketose as the receptor. The enzyme is characterized by:

Method for the preparation of fructose-containing oligosaccharide

In a method for preparing multiple glucosyl branched-cyclodextrins, a debranching enzyme is contacted to a mixture of maltose or a substance containing maltooligosaccharide such as maltose with a cyclodextrin to obtain a reaction product from which a branched-cyclodextrin is then separated; said branched-cyclodextrin is converted to glycosyl-cyclodextrin under the action of glucoamylase, which is thereafter mixed with maltose or a substance containing maltooligosaccharide such as maltose; and the resulting mixture is successively acted on by a debranching enzyme and glucoamylase.

Method for the preparation of multiple glucosyl branched-cyclodextrins

The invention provides a method for pulverizing or granulating nuts or seeds which comprises mechanically grinding a mixture of nuts or seeds with a cyclodextrin. The invention also provides a stable particulate admixture of cyclodextrin containing nuts or seeds. The admixture is highly stable during storage and provides a versatile product for use in food processing.

Method for the pulverization or granulation of nuts and seeds

Growing community of inventors

Yokohama, Japan

Kozo Hara