Georgia Tech Research Corporation

Emory University

Kousik Kundu

Niren Murthy

W Robert Taylor

Sarah F Knight

Sungmun Lee

The present disclosure is generally related to methods of preventing oxidative stress injury induced by renal ischemia-reperfusion by a Folate-TEMPOL conjugate.

Methods for preventing oxidative stress injury of a tissue by a Folate-TEMPOL conjugate

Reduced dyes, such as hydrocyanines, deuterocyanines, and/or other deuterated dyes capable of detecting one or more reactive oxygen species are described herein. The reduced dyes exhibit little or no fluorescence due to the disrupted &#x3c0; conjugation. However, upon reaction with ROS, the reduced dyes are oxidized, regenerating the extended &#x3c0; conjugation and causing a substantial increase in fluorescence intensity. In many case, the oxidized dye is generally membrane impermeable. However, upon reduction, many of the reduced dyes are membrane permeable. Thus, reduced dyes can accumulate in cells and/or tissue to amplify the signal. Once inside the cell or tissue, the reduced dye is reoxidized upon reaction with ROS, and the oxidized dye again becomes membrane impermeable, trapping the dye within the cell. The reduced dyes can be used to image ROS, such as hydroxide radical and superoxide, in serum, cell cultures, tissue explants, and in vivo.

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Atlanta, GA, United States of America

Kousik Kundu