Jsr Corporation

The University of Western Ontario

Jsr Life Sciences Corporation

Kouji Tamori

Satoshi Nakamura

Tetsuo Fukuta

Eiji Takamoto

Yusuke Okano

Yong Wang

Takayoshi Abe

Masaki Momiyama

Toshihiro Ogawa

Mitsuhiro Murata

Takahiro Kawai

Satoshi Katayose

Masaaki Miyaji

Tomonori Shiotani

Masaru Ueno

Kiyoshi Kasai

Kenji Ishizuki

Hiroshi Kawai

Xing Li

Satoshi Hyugaji

Shun-Cheng Li

Yu Otani

Courtney Voss

Takuo Sone

Kinji Yamada

Hiroshi Shiho

Naoki Hayashi

Akira Nishikawa

Hirotaka Shida

Masayuki Takahashi

Tokio Sawai

Katsuhiko Tsuruoka

Takeshi Mogi

Yasuhisa Watanabe

Hirokazu Kai

Satoshi Futami

Kejun Fan

Kazuhiro Ikkyu

Yingjia Xu

Shin-ya Omote

Tomohiro Uetsuhara

Yuusuke Okano

Ryou Tsuda

Sachiko Tsuda

Shin-Ya Omote

Provided are an additive and a surface treatment agent capable of suppressing agglutination of latex particles contained in a reagent for a latex agglutination reaction during storage of the reagent although a synthetic polymer is contained as an active component. An additive is to be added to latex particles used in a reagent for a latex agglutination reaction. The latex particles have not been subjected to blocking treatment. The additive includes a polymer containing more than 60% by mass and 99% by mass or less of hydrophilic repeating units (A) relative to all repeating units and 1% by mass or more and less than 40% by mass of hydrophobic repeating units (B) relative to all repeating units, and having a weight average molecular weight of 3,000 or more.

Additive, surface treatment agent, surface-modified latex particles, method for producing surface-modified latex particles, reagent for latex agglutination reaction, kit, and method for detecting target substance

To provide a solid-phase carrier that has extremely low nonspecific adsorption of biological substances such as proteins, peptides, nucleic acids and cells, and is capable of maintaining the activity of bound ligands at a high level; and a method for capturing a target substance using the solid-phase carrier. A method for capturing a target substance, including: a step for preparing a solid-phase carrier for capturing a target substance, the carrier having a base material, at least a portion of the surface thereof being formed of or coated with a saccharide, and having a linker of 5-100 atoms having a reactive functional group for binding a ligand that specifically binds to the target substance, wherein the saccharide is chemically bound to the linker; a step for binding the ligand to the solid-phase carrier to obtain a solid-phase carrier having a ligand bound thereto; and a step for bringing the solid-phase carrier having the ligand bound thereto into contact with a sample containing the target substance that specifically binds to the ligand.

Method for capturing target substance, solid-phase carrier for capturing target substance, and method for producing solid-phase carrier

A process for producing carrier polymer particles comprising covering the surface of organic polymer particles having a particle diameter of 0.1 to 20 micrometers with a saccharide by chemically bonding the organic polymers and the saccharide.

Carrier polymer particle, process for producing the same, magnetic particle for specific trapping, and process for producing the same

To provide a method for producing polymer particles from an acrylamide monomer by a normal phase suspension polymerization method, polymer particles, a packing material for a chromatography column using the particles, and a chromatography column. A method for producing polymer particles, comprising dispersing a monomer composition comprising a multifunctional monomer having a plurality of N-substituted acrylamide groups in an aqueous medium; and polymerizing the dispersed monomer composition.

Method for producing polymer particles, polymer particles, filler for chromatography column, and chromatography column

Provided is a filler for affinity chromatography having a high dynamic binding capacity for proteins and having excellent alkali resistance and storage stability. The filler for affinity chromatography of the present invention comprises a porous particle consisting of a copolymer of 40 parts to 99.5 parts by mass of (M-1) a methacryloyl group-containing vinyl monomer that contains a hydroxyl group and does not contain an epoxy group, 0.5 parts to 30 parts by mass of (M-2) an epoxy group-containing vinyl monomer, 0 parts to 59.5 parts by mass of (M-3) a methacryloyl group-containing vinyl monomer which is other than the monomers (M-1) and (M-2), and 0 parts to 25 parts by mass of (M-4) a vinyl monomer other than the monomers (M-1), (M-2) and (M-3) (with the proviso that the total amount of the contents of (M-1), (M-2), (M-3) and (M-4) is 100 parts by mass); ring-opened epoxy group obtainable by ring-opening of epoxy group that is contained in the copolymer; and ligand that is bound to the porous particle.

Filler for affinity chromatography

A nonspecific adsorption inhibitor of a substance relating to a living body, which inhibits nonspecific adsorption of a substance relating to a living body such as various species of proteins which are used in clinical diagnostic agents, clinical diagnosis devices, biochips and the like, and a method for coating an article using said nonspecific adsorption inhibitor.

Nonspecific adsorption inhibitor of substance relating to living body and method for coating article

Provided is a filler for affinity chromatography which is useful for protein purification and contains porous particles that have a high dynamic binding capacity for proteins and excellent pressure characteristics. The filler for affinity chromatography of the present invention is characterized in that it includes a porous particle consisting of a polymer of vinyl monomer including a cross-linkable vinyl monomer that contains hydroxyl group but does not contain epoxy group and an epoxy group-containing non-cross-linkable vinyl monomer, or a cross-linkable vinyl monomer that contains hydroxyl group and epoxy group, ligands bound to the porous particle, and ring-opened epoxy groups.

The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.

Alkali-resistant variants of protein A and their use in affinity chromatography

Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier.R&#x2014;R&#x2003;&#x2003;(1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and Rrepresents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R.

Filler for affinity chromatography and method for isolating immunoglobulin

Provided are a support for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. A support for affinity chromatography, containing an immobilized protein ligand represented by the following formula (1):R&#x2014;R&#x2003;&#x2003;(1)

Support for affinity chromatography and method for isolating immunoglobulin

A non-specific adsorption inhibitor includes a water-soluble polymer obtained by polymerizing a monomer unit that includes a monomer (A) that forms a polymer that has a lower critical solution temperature (LCST) of 80&#xb0; C. or less in an aqueous solution under normal pressure when subjected to homopolymerization, and a monomer (B) that forms a polymer that does not have the LCST in an aqueous solution under normal pressure when subjected to homopolymerization.

Non-specific adsorption inhibitor

An affinity chromatography packing material includes porous mother particles that include a copolymer of a monomer mixture including a crosslinkable vinyl monomer and an epoxy group-containing vinyl monomer, a ligand being bound to the porous mother particles, and the porous mother particles including a ring-opening epoxy group produced by ring-opening of the epoxy group included in the porous mother particles.

Packing material for affinity chromatography

The organic polymer particles comprise a carboxyl group and 2,3-dihydroxypropyl group, and the magnetic particles for diagnostics comprise fine magnetic material particles and a polymer part containing a hydrophilic polymer part and a crosslinked polymer part, a dry coating film obtained from a water dispersion thereof having a contact angle with water of 5&#xb0; to 60&#xb0;. The process for producing the carboxyl group-containing particles comprises a step of producing an ester bond by reacting a hydroxyl group in organic polymer particles having the hydroxyl group with a carboxylic anhydride. The organic polymer particles comprise a hydroxyl group originating from a 2,3-dihydroxypropyl group and a polyoxyethylene group.

Organic polymer particles and process for producing the same, magnetic particles for diagnostics, carboxyl group-containing particles and process for producing the same, and probe-bound particles and process for producing the same

A method for producing a non-specific adsorption inhibitor includes reacting (A) a tosylated compound of polyoxyethylene monomethyl ether with (B) a polyamine having either an amino group or imino group (&#x2014;NH&#x2014;), or both, in total of 3 to 12.

Non-specific adsorption inhibitor, probe-bonded particles, and method for producing the same

A non-specific adsorption inhibitor of formula (1), wherein n is a real number of 4 to 2,000, Rand Rindividually represent a hydrogen atom or a group comprising 1 to 11 amino group(s) or imino group(s), or both, provided that the total number of amino groups and imino groups contained in Rand Ris 2 to 11. Non-specific adsorption inhibitor composition, probe-bonded particles.

Growing community of inventors

Tsuchiura, Japan

Kouji Tamori