Agency of Industrial Science and Technology

Extra Ministerial Bureau of Ministry of International Trade and Industry

Kouichi Kawamura

Masaru Honjo

Yoshio Furutani

Akira Nakayama

Hiroaki Shimada

Izumi Mita

Akiko Akaoka

A expression-secretion vector is constructed by using a DNA sequence comprising a DNA fragment obtained by subjecting a DNA sequence consisting of a region involved in the expression of a gene coding for an extracellular enzyme of a bacterium of the genus Bacillus and a region involved in the secretion of the enzyme thus expressed to a deletion in the region involved in the secretion which can enhance the extracellular production of a protein dependent on the DNA fragment. By transforming a host bacterium with a recombinant DNA molecule formed by inserting a DNA fragment comprising a gene coding for a desired protein into the expression-secretion vector and then culturing the transformed host, the extracellular production of the desired protein in a large amount can be accomplished.

DNA sequence involved in gene expression and protein secretion,

Growing community of inventors

Mobara, Japan

Kouichi Kawamura