Japan Science and Technology Agency

Kosuke Nakamoto

Hiroshi Abe

Hiroki Murase

Yasuaki Kimura

Naoko Abe

A primer for amplifying a nucleic acid having a structure represented by the formula (1): where B represents a base, Rrepresents a decomposable protecting group, and Rrepresents hydrogen or a hydroxyl group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA, includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3'-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; and a photoirradiation unit for deprotecting Rand forming a sticky end with a 3′-protruding end.

Primer, device for producing double-stranded DNA using primer, and method for producing double-stranded DNA using primer

where Arepresents —S—, —S—S—, or —Se—, B represents a base, and Rrepresents a decomposable protecting group, and the symbol * represents a bond to a sugar of an adjacent nucleotide. A device for producing double-stranded DNA includes: a forward primer and a reverse primer, having a structure represented by formula (1); a PCR device for forming double-stranded DNA with 3'-recessed ends by performing multiple cycles of PCR by using a template DNA as a template; Klenow fragment for making the 3′ ends blunt; and a photoirradiation unit for deprotecting Rand forming a sticky end with a 3′-protruding end.

Growing community of inventors

Nagoya, Japan

Kosuke Nakamoto