National Institute of Advanced Industrial Science and Technology

Daiichi Sankyo Company, Limited

Kyowa Hakko Kirin Co., Limited

Kosuke Kuroda

Kazuo Kobayashi

Yasunori Chiba

Kimihisa Ichikawa

Yoshifumi Jigami

Koichi Nonaka

Takeshi Suzuki

Yoshie Takahashi

Minako Ono

This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.

Method for high-level secretory production of protein

This invention provides a means for enabling high-level secretory production of proteins, in particular proteins having complicated structures such as antibodies, in host cells such as yeast cells. The invention also provides transformed yeast cells having the activated HAC1 gene and the RRBP1 gene and a method for enabling high-level secretory production of foreign proteins using such transformed host cells by inhibiting O-sugar chain formation indigenous to host cells such as yeast cells.

Growing community of inventors

Takasaki, Japan

Kosuke Kuroda