Life Technologies Corporation

Life Technologies as

Invitrogen Dynal as

Kornelija Zgonc

Darren Ellis

Tine Borgen Hildisch

Richard Johan Toften

Erlend Ragnhildstveit

Espir Kahatt

Lars Nokleby

Gaute Brede

Timothy Updyke

Joseph Amshey

Todd Peterson

Michael Thacker

Alexander Khorlin

Timothy Powers

Daniel Bezdek

Lance Parsons

Mark R Andersen

Michael C Pallas

Jian Gong

Marcin Sikora

Gordon A Janaway

Casey R McFarland

Haopeng Wang

Gothami Padmabandu

Jamie Hogan

Ferrier Le

Lars Norderhaug

Tommy Rivrud

Jeremy Mundy

Morten Egeberg

Jeremy (“Jez”) Mundy

Gil Cavada

Lars Noekleby

Jez Mundy

Jeremy (Jez) Mundy

Lars Nøkleby

Malin Karlsson

Norman Trolson

Malin Karlson

Casey Mcfarland

Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

System and method for determining copies-per-unit-volume using PCR and flow control of droplets

The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

Apparatus for and method of processing biological samples

The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

Magnetic separation apparatus

The present disclosure relates to a magnetizing portion for providing a high-gradient magnetic field in a magnetic separation device. The magnetizing portion comprises at least one magnetic assembly. The at least one magnetic assembly comprises: a plurality of magnets whereby each magnet has a north pole, south pole and a magnet axis extending between the north and south poles, and the plurality of magnets are arranged one above the other in a direction at least substantially perpendicular to the axis of each magnet in such a manner that the north and south poles of adjacent magnets are arranged alternately and a space is provide between adjacent magnets; and at least one non-magnetic spacing means arranged in the space between adjacent magnets. The present disclosure also relates to magnetic separation devices comprising at least one of the said magnetizing portions and to a method of isolating magnetically labelled particles using the magnetic separation devices.

Magnetising portion for a magnetic separation device

The disclosure relates to a magnetic separation rack for isolating magnetically labeled particles from a non-magnetic medium comprising a body portion () and a foot portion (). The body portion comprises an array of sample vessel retaining portions () and plurality of magnetizing portions (). Each sample vessel retaining portion comprises at least one visible portion such that when a sample vessel is mounted in a sample vessel retaining portion at least one portion of the sample vessel is visible to a user. The magnetizing portions are arranged within the body portion () such that at least two magnetizing portions () are circumferentially spaced about each sample vessel retaining portion (). The foot portion is pivotally coupled to the body portion such that the body portion is operatively tiltable with respect to the foot portion such that each sample vessel retaining portion may retain a sample vessel mounted therein in a tilted position with respect to the vertical. The disclosure further relates to a method of isolating magnetically labeled particles from a non-magnetic medium using the said magnetic separation rack.

Magnetic separation rack

Provided herein is a bioprocessing device, bioprocessing card, and fluidics cartridge for performing automated bioprocessing of a sample. The bioprocessing card may include a plurality of pipette tips; and at least one pump in fluid communication with the plurality of pipette tips. In some embodiments, the pumps and the pipette tips are in fluid communication through a processing channel which may be a microscale channel. Also provided herein is an automated bioprocessing device comprising: at least one bioprocessing card; at least one fluidic cartridge; and an automated control system configured to control automated bioprocessing of a sample. Further provided herein are methods of use of the device, card, and cartridge.

Apparatus for and method of automated processing of biological samples

The disclosure relates to a magnetic separating device for isolating magnetically labelled particles from a non-magnetic medium comprising a body portion () having a magnetising portion () for providing a magnetic field and a surface by means of which the body portion may stand on a supporting surface; and a sample vessel retaining portion () for retaining at least one sample vessel (), wherein, the magnetising portion () is configured to conform at least approximately to at least a substantial portion of the longitudinal profile of at least one sample vessel (); the sample vessel retaining portion () is configured to retain at least one sample vessel () such that at least a portion of the contents of the sample vessel () is visible to a user; and the sample vessel retaining portion () is configured to be mountable on the magnetising portion so that in use, the at least one sample vessel is subject to the magnetic field of the magnetising portion (). The disclosure further relates to a kit of parts and a method for isolating magnetically labelled particles from a non-magnetic medium using the magnetic separation device.

Magnetic separating device

Laboratory apparatus

Growing community of inventors

Carlsbad, CA, United States of America

Kornelija Zgonc