Applied Biosystems, LLC

Life Technologies Corporation

Konrad Faulstich

Jun Xie

Debjyoti Banerjee

Umberto Ulmanella

Aldrich Lau

Aldrich N K Lau

Dar Bahatt

Vladimir Ivanovich Bashkirov

Aldrich NK Lau

Kristian M Scaboo

Disclosed, for example, are methods comprising cleaving an uncleaved probe to form a cleaved oligonucleotide flap, forming a hybridization complex between the cleaved oligonucleotide flap, a bridging oligonucleotide, and a capture oligonucleotide that is immobilized on a surface, such that the oligonucleotide flap and the capture oligonucleotide are hybridized to immediately adjacent, complementary regions of the bridging oligonucleotide, ligating the oligonucleotide flap to the capture oligonucleotide to form an immobilized ligation product, and detecting the ligation product.

Electrochemical polynucleotide detection comprising ligation

A diagnostic device is provided that includes a plurality of retainment regions interconnected through at least one fluid processing passageway or separated by at least one barrier. A fluid flow modulator can be provided in the fluid processing passageway if a fluid processing passageway is provided. The barrier and/or fluid flow modulator can comprise a polysaccharide, a derivative of a polysaccharide, or a combination thereof. For example, the barrier can comprise a chitosan material.

Fluid processing device including size-changing barrier

The present teachings provide a detection cell for a biological material and methods for detecting biological material including a photosensitive material optically coupled to an interior volume containing the biological material so to avoid optical components or an external light source.

Light collection system

A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.

Microfluidic device including a dissolvable structure for flow control

A fluid processing device is provided that includes a substrate, a plurality of fluid retainment regions formed in or on the substrate, and a barrier at least partially separating two or more of the fluid retainment regions. The barrier includes a mixture of a sequestering material and a reaction component. The reaction component can be at least one of a reactant, a reagent, a catalyst, an initiator, a promoter, a cofactor, an enzyme, a salt, or a combination thereof. The sequestering material can be a porous material, a dissolvable material, or both.

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Salem-Neufrach, Germany

Konrad Faulstich