Toyo Kohan Co., Ltd.

Other

Nihon Parrkerizing Hiroshima Co., Ltd

Nihon Parkerizing Hiroshima Co., Ltd.

Kojiro Takahashi

Michifumi Tanga

Osamu Takai

It is intended to provide a method of reusing a DNA-immobilization substrate whereby the expensive DNA-immobilization substrate can be efficiently utilized and a reusable DNA-immobilization substrate having the same performance as a new product without any trouble in practical use can be provided. Namely, a method of reusing a DNA-immobilization substrate characterized in that, to remove DNA from a DNA-immobilization substrate carrying the DNA immobilized by an acid-amide bond via an oligonucleotide to thereby enable the immobilization of a fresh DNA, the acid-amide bond between the substrate and the DNA is hydrolyzed with an acid or an alkali.

Method of reusing DNA-immobilization substrate

Method and apparatus for constructing a cDNA library by hybridizing mRNA with oligo (dT) on a support and treating with a reverse transcriptase to immobilized complementary DNA, or for constructing a gDNA library by ligating a double-stranded chromosomal DNA library with an oligonucleotide on a support having a restriction enzyme site and then immobilizing the gDNA library.

Methods for constructing DNA library and support carrying DNA library immobilized thereon

Substrates for producing a DNA library are produced by immobilizing DNA onto a suitable substrate having excellent thermal conductivity. A surface of the substrate is chemically modified with a radical having a terminal polar radical. DNA can be amplified by the PCR method in a short period of time using substrates of the present invention onto which DNA is immobilized.

Substrates for immobilizing and amplifying DNA and DNA-immobilized chips

Supports for immobilizing DNA or the like

A purpose of the present invention is to provide a gene amplification apparatus with simple operation, wherein the apparatus can heat and cool gene rapidly and a thermal cycle can be operated in a relatively short period and a method thereof, a method for preparing immobilized DNA library suitable to the above apparatus and a method for comparing genes systematically. The apparatus for preparing immobilized DNA library according to the present invention comprises a reaction body on which a grooved portion for receiving a container is formed, a cap portion provided at an upper portion of the reaction body and a container formed by substrates for immobilizing DNA, wherein the cap portion including means for heating/cooling and means for cooling

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Hiroshima-ken, Japan

Kojiro Takahashi