Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo

Director-gen. of the Tea Experiment Station, Ministry of Agriculture and

Other

Misaki, Akira

Koji Yokobayashi

Akira Misaki

Toshiyuki Sugimoto

Tadashi Ikeda

Yoichi Tsuburaya

Shigeo Takaya

Products such as film, sheet, paper, fiber, coating material, binder, and molded materials e.g. capsules, cups et al are disclosed. The products are prepared by use of an alpha-glucan comprising repeating units of [3)-Glc-(1.fwdarw.4)-Glc-(1.fwdarw.4)-Glc-(1.fwdarw.], (wherein Glc represents alpha-D-glucopyranose residue) as its sole constituent or a member of its constituents according to conventional methods.

Shaped products of alpha-glucan

A glucan which is characterized by repeating units of [3)--Glc--(1.fwdarw.4)--Glc--(1.fwdarw.4)--Glc--(1.fwdarw.] wherein Glc represents alpha-D-glucopyranose residue. Said glucan may be prepared by cultivating a microorganism of the genus Elsinoe capable of producing said glucan on a nutrient medium to produce said glucan and separating and recovering said glucan.

Glucan and a process for the production thereof using Elsinoe

A glucan which is characterized by repeating units of [3)-Glc-(1.fwdarw.4)-Glc-(1.fwdarw.4)-Glc-(1.fwdarw.] wherein Glc represents alpha-D-glucopyranose residue. This glucan may be prepared by cultivating a microorganism of the genus Elsinoe capable of producing this glucan on a nutrient medium to produce this glucan and separating and recovering this glucan. This glucan may be used, for example, in the form of a film for packaging film material.

Glucan polysaccharide

A glucan characterized by repeating units of [3)-Glc-(1.fwdarw.4)-Glc-(1.fwdarw.4)-Glc-(1.fwdarw.] wherein Glc represents alpha-D-glucopyranose residue is produced by cultivating a microorganism of the genus Elsinoe capable of producing said glucan on a nutrient medium containing one or more members selected from the group consisting of various starch hydrolyzates, mannose, fructose, mannitol and xylose to produce said glucan in the medium, separating and recovering said glucan therefrom.

Processes for producing glucan using Elsinoe

A culture medium composed of necessary sources and tap water is sterilized, inoculated under strains of the genus Streptococcus and then cultivated under aeration and agitation. Subsequent to the cultivation, the resultant was subjected to water-washing and sieving to obtain crude glucan. The crude glucan is then dissolved in NaOH solution and centrifuged. The recovered filtrate is neutralized with HCl and then the insolubilized glucan was collected by centrifugation. After repetition of the procedures, the collected glucan is washed with water thoroughly until no chloride ion is detected in the waste water and then dried to form white purified glucan powder.

Process for the removal of sucrose from a sugar mixture

A culture medium composed of carbon source, nitrogen source and minerals was sterilized at 120.degree. for 15 minutes, inoculated with strains of the genus Streptococcus and then cultivated at 35.degree. C for 72 hours under aeration and agitation. Subsequent to the cultivation, the resultant was subjected to water-washing and sieving to obtain crude glucan. The crude glucan was then dissolved in NaOH solution and centrifuged. The recovered filtrate was neutralized with HCl and then the insolubilized glucan was collected by centrifugation. The collected glucan was washed with water thoroughly until no chloride ion was detected in the waste water and then dried to form white purified glucan powder.

Growing community of inventors

Okayama, Japan

Koji Yokobayashi