Asahi Kasei Kabushiki Kaisha

Koji Maehana

Kenji Matsuyama

Takamitsu Utsumi

An object of the present invention is to elucidate the cause of degradation of the reliability of detecting causative bacteria of environmental mastitis such asbacteria,bacteria, and CNS, and to provide a highly reliable detection means. Another object of the present invention is to provide a highly reliable detection means based on the immunochromatographic method, which enables quicker determination compared with the cultivation-based method, and a diagnosis kit using it. The present invention provides a method for detecting a causative bacterium of environmental mastitis of a livestock animal, which comprises: the step of determining whether number of the causative bacterium in a milk of a subject livestock animal is not smaller than a determination value defined beforehand on the basis of distribution of amounts of the causative bacterium in milks derived from a non-disease group and distribution of the amounts of causative bacterium in milks derived from a disease group by an immunological test method, and wherein the immunological test method is an immunochromatographic method using an antibody labeled with not less than 0.6/cmand not more than 3.5/cmof gold colloid particles (particle-labeled antibody).

Method for detecting causative bacterium of mastitis

The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacteria of mastitis are coliform bacteria or not by using milk of a livestock animal. There is provided a method for lysing coliform bacteria, which comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of anionic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse coliform bacteria existing in the milk. The lytic enzyme is preferably lysozyme.

Method for detecting coliform bacteria contained in milk

The object is to provide a lysis method and lysis treatment solution for efficiently lysing cells of variousbacteria in milk of a livestock animal to release a specific antigen substance contained in the cells for detecting whether causative bacterium of mastitis is abacterium or not by using the milk, as well as a detection method using an immunochromatographic device. There is provided a method for lysing abacterium, which comprises the step of mixing a lysis agent containing a lytic enzyme with milk obtained form a livestock animal to lyse abacterium existing in the milk. The lytic enzyme is preferably at least one selected from the group consisting of lysozyme, labiase, and &#x3b2;-N-acetylglucosaminidase.

Method for detectingbacterium contained in milk

The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacterium of mastitis is aor not by using milk of a livestock animal. There is provided a method for lysing a, which comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of ampholytic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse aexisting in the milk. The lytic enzyme is preferably lysostaphin.

Method for detectingcontained in milk

An immunochromatographic method for detecting a specific substance contained in milk, which comprises (1) the step of contacting the milk with a test strip having a first part retaining a labeled first antibody directed to the specific substance or the specific substance that is labeled, a second part disposed downstream from the first part, on which a second antibody directed to the specific substance is immobilized, and a third part disposed upstream from the first part or the second part and having voids enabling removal of milk fat globules contained in the milk, at the third part or a further upstream part, and (2) the step of flowing the milk up to the second part or a further downstream part to obtain a detectable signal of the label at the second part or a further downstream part.

Method for detecting specific substance in milk

The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacterium of mastitis is aor not by using milk of a livestock animal. There is provided a method for lysing awhich comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of ampholytic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse aexisting in the milk. The lytic enzyme is preferably lysostaphin.

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Tokyo, Japan

Koji Maehana