Two Cells Co., Ltd.

Other

Gc Corporation

Japan Science and Technology Agency

Hiroshima University

Kato, Yukio

Koichiro Tsuji

Yukio Kato

Jin Chang Shao

Katsuyuki Yamanaka

Yuki Katsura

Yasufumi Takahashi

Masato Takao

Takashi Matsushita

Akira Igarashi

Masahiro Nishimura

Masayoshi Ono

Takeshi Kawamoto

Tomohisa Okura

Mika Takeda

Yuhiro Sakai

Toshitsugu Kawata

Yoshie Ozaki

Kaoru Tenjo

Maiko Hara

Minoru Morikawa

Satoshi Maeda

Yui Kitayama

Aki Ohmori

Shinichi Hasegawa

Shinya Miki

Ken Innami

Toshie Tsuchiya

Masakazu Shimizu

Takao Masaki

Ken Yoshida

Yukio c/o Hiroshima University Kato

Chika University Of Toyama Graduate School Of Med And Pharm Sc Koike

A method according to the present invention for producing a cartilage-damage treatment agent, includes the steps of: (i) proliferating mesenchymal stem cells in a serum-free medium A containing an FGF, a PDGF, a TGF-&#x3b2;, an HGF, an EGF, at least one phospholipid and at least one fatty acid; and (ii) mixing the mesenchymal stem cells thus proliferated in the step (i), an isotonic preserving agent, and a cytoprotective agent. This method provides a cartilage-damage treatment agent which favorably regenerates a cartilage tissue.

Cartilage-damage treatment agent and method for producing same

Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof

Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and &#x3b2;-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.

Differentiation-inducing culture medium additive and use thereof

A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium 'A' containing an FGF, a PDGF, a TGF-&#x3b2;, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).

Cell preparation containing mesenchymal stem cells, and method for producing same

Disclosed is a method for distinguishing a mesenchymal stem cell comprising, using at least one gene selected from the genes having the nucleotide sequences indicated by the accession numbers shown in Table 1 as a distinguish marker, detecting the difference in expression of the distinguish marker between a mesenchymal stem cell and a connective tissue cell to distinguish the mesenchymal stem cell from the connective tissue cell. This method enables to distinguish an undifferentiated mesenchymal stem cell from other connective tissue cell such as fibroblasts, osteoblasts, chondrocytes and adipose cells with good accuracy. A mesenchymal stem cell given by this method or a composition comprising the mesenchymal stem sell can be used as a therapeutic for use in the regenerative medicine.

Method for distinguishing mesenchymal stem cell using molecular marker and use thereof

Method for regeneration of mesenchymal tissue from mesenchymal cells on a porous sheet composed of bioabsorbable polymer material.

Sheet for guiding regeneration of mesenchymal tissue and production method thereof

This invention provides therapeutic agents, transplants and therapeutic methods that can enhance the regeneration of injured tissue. This invention relates to agents, transplants and therapeutic methods for enhancing the migration and accumulation of mesenchymal stem cells in injured tissues and/or suppressing the diffusion of mesenchymal stem cells from injured tissues.

Therapeutic agents and therapeutic methods for treating injured tissue

To efficiently select and proliferate the mesenchymal stem cells without necessity of an exclusive separating device and a complicated separating operation, mesenchymal stem cells are cultured by seeding at least one of a bone marrow solution, an umbilical cord blood, a peripheral blood, a synovial membrane and an amniotic membrane in a liquid culture medium which is filled in a vessel, includes water as its main components and having a specific gravity between 1.06 and 1.10 at 37&#xb0; C., and making a culture at a temperature 37&#xb1;2&#xb0; C. on a ceiling side surface of the vessel, preferably the specific gravity being regulated by use of at least one selected from silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound including a triiodo aromatic ring.

Culturing method of mesenchymal stem cell

A thrombus control agent comprising a human granulocyte colony stimulating factor (human G-CSF) as an active ingredient and a pharmaceutically acceptable carrier, a method of treating thrombi by administering the thrombus control agent to a patient bearing thrombi formed in the arterial and venous vessels, and use of human G-CSF for preparing the thrombus control agent are disclosed. The inventors found that human G-CSF is useful for treating thrombi and developed the use of human G-CSF for this purpose although nobody has clearly reported this type of pharmacological activity of human G-CSF.

Method of treating thrombi with G-CSF

Growing community of inventors

Hiroshima, Japan

Koichiro Tsuji