Other

Sumitomo Chemical Company, Limited

Gakko Hojin Kinki Daigaku (educational Foundation Kinki Univ.)

Koichiro Komai

Shunichi Shiozawa

Iwao Nakatsuka

Hideo Kaneko

Koichi Saito

Masaki Mori

Yoshiyasu Yabusaki

Junichi Iwamura

Toshiyuki Sakaki

Koji Hayashi

Hirofumi Yagi

Masao Hirose

Mikiko Nakatsukasa

Nao Matsuura

An gene was identified as a RA disease susceptibility gene on Human Chromosome 8, the gene coding a protein that has an amino acid sequence shown in SEQ. ID NO. 1 and that has such mutation that glycine is inserted as a 269th amino acid in the sequence. Moreover, it was found that mutation of the gene and the protein relate to onset of RA. Achieved is a method of evaluating with high accuracy the onset or onset possibility of RA by using the mutation.

Disease susceptibility gene for rheumatoid arthritis, protein thereof, evaluation method and evaluation kit for evaluating onset possibility of rheumatoid arthritis by using those, and remedy and curing medicine for rheumatoid arthritis

A genomic DNA involved in rheumatoid arthritis, a method of diagnosing rheumatoid arthritis or a method of judging onset risk of rheumatoid arthritis, and a diagnostic kit for diagnosing rheumatoid arthritis or judging onset risk of rheumatoid arthritis, which genomic DNA comprises at least one of the following mutations in genomic DNA consisting of the base sequence of SEQ ID NO: 1:

Genomic DNAs participating in rheumatoid arthritis, method of diagnosing the same, method of judging onset risk and diagnostic kit for detecting the same

As a disease gene for rheumatoid arthritis present in human chromosome X and a method of diagnosing rheumatoid arthritis, a disease gene, which is a mutant of protooncogene Dbl transcribing an mRNA that encodes the cDNA of which the sequence from the 2679th to 2952nd bases is shown in SEQ ID NO: 1, which disease gene transcribes an mRNA encoding the cDNA of which the region from the 20th to 274th bases in SEQ ID NO: 1 is substituted with the sequence of SEQ ID NO: 2, and a method for diagnosing rheumatoid arthritis by detecting the mRNA of the above-described gene or its expression product in a biological specimen, is provided.

Rheumatoid arthritis gene

There is disclosed a method for evaluation of the safety of a chemical compound, which includes the steps of: (a) reacting a chemical compound with recombinant yeast cells expressing, or in other words producing, human cytochrome P450 molecular species P450 1A2, P450 2C9, P450 2E1 and P450 3A4 together with a yeast NADPH-P450 reductase, which may be in the form of a fused enzyme with each of said human cytochrome P450 molecular species, or with the cell free extracts of the yeast cells; and (b) analyzing the resulting metabolite to determine the safety of the compound. According to this method, it can be determined whether a test compound will be converted into a carcinogenic or mutagenic form through the metabolism in the human liver, and whether the test compound or its metabolite has mutagenicity.

Method for safety evaluation of chemical compound using recombinant yeast expressing human cytochrome P450

Diagnostic kit

Oligonucleotide for use in checking presence or absence of mutation in

A plant growth regulating composition which comprises as an active ingredient at least one diterpene glucoside of the formula: ##STR1## wherein R.sup.1 is a hydrogen atom or any other cation and R.sup.2 is .beta.-glucopyranosyl-(1-2)-.beta.-glucopyranosyl or 2,3-di-O-(.beta.-glucopyranosyl-.beta.-glucopyranosyl, or R.sup.1 is .beta.-glucopyranosyl and R.sup.2 is .alpha.-rhamnopyranosyl-(1-2)-.beta.-glucopyranosyl, .beta.-glucopyranosyl-(1-2)-.beta.-glucopyranosyl, 2,3-di-O-(.beta.-glucopyranosyl)-.beta.-glucopyranosyl or (3-O-.beta.-glucopyranosyl)-(2-O-.alpha.-rhamnosyl-.beta.-glcopyranosyl).

Plant growth regulator

Growing community of inventors

Hyogo, Japan

Koichiro Komai