Shimadzu Corporation

Koichi Yamagata

Yoshinari Shirasaki

Tetsuo Ohashi

Shigeru Fukushima

Jun Tada

Naoyuki Nishimura

Hiroko Ozaki

Hirohisa Abe

Hiroyuki Jikuya

Yoshihiro Aoyama

Jun Takano

A method for testing causative bacterial species of food poisoning which is characterized by using two oligonucleotide primers that hybridize to opposite strands of bacterial DNA specifically, and flank a unique region in the target DNA and amplifying the specific fragment of the bacterial DNA, comprising the steps of:

Method for testing causative microorganisms of food poisioning and

Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

Oligonucleotides for detecting bacteria and detection method using same

Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

Oligonucleotides for detecting bacteria

The method of capillary electrophoresis of the present invention is carried out using an electrophoretic buffer containing an ungelled agarose polymer, wherein the agarose polymer concentration may be constant or may be changed on a time basis. The method of the present invention permits efficient fractional assay with high reproductibility. Particularly, the mode using the apparatus of the present invention in which the agarose polymer concentration is changed on a time basis is suitable for fractional assay of DNA and proteins of a wide range of size because an electrophoretic buffer having an ideal composition for separation is supplied constantly.

Method of capillary electrophoresis

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Osaka, Japan

Koichi Yamagata