Shimadzu Corporation

Omron Corporation

Omron Healthcare Company Ltd.

Hiroshima University

Kanzaki Paper Manufacturing Co., Ltd.

Otsuka Pharmaceutical Factory, Inc.

The Noguchi Institute

Osaka Prefectural Hospital Organization

Kabushiki Kaisha Nisshin Seisa Kusho

Koichi Tanaka

Norihito Yamamoto

Yuko Fukuyama

Tetsuya Sato

Shigeki Kobayashi

Shunsuke Izumi

Yukiya Sawanoi

Takayuki Nakamura

Atsushi Hisano

Kentaro Mori

Feilang Tseng

Yoshitake Oshima

Takashi Shimada

Kuniyoshi Takahashi

Hiromi Wada

Taka-Aki Sato

Tamio Miyake

Akitoshi Miki

Shigeru Tanimura

Kiichiro Miyata

Takushi Yamamoto

Yoshinao Wada

Junko Amano

Kazuhito Murata

Akira Okushi

Shigeharu Myogatani

Akiyo Kinoshita

Yoshio Nakae

Koichi Kojima

Yoshitsugu Hamaguchi

Manaho Yamaguchi

Yuzo Yamazaki

Xiangdong Zhao

Takaaki Koshiba

An analysis method incudes: preparing a sample containing microorganisms; placing the microorganisms under a first condition and then performing a first mass spectrometry on a substance produced by the microorganisms; and obtaining information on characteristics or classifications of the microorganisms contained in the sample on a basis of a difference between a first data obtained in the first mass spectrometry and a second data obtained when a second mass spectrometry is performed on a substance produced by the microorganisms after having been placed under a second condition as well as a reference data in which classifications or characteristics of the microorganisms are associated with the data obtained in the mass spectrometry of the microorganisms, in which the first condition and the second condition differ in a sugar concentration or an oxygen concentration in an environment in which the microorganisms are placed.

Analysis method, microorganism identification method, and testing method

One mode is a method for the structural analysis of an organic compound by MALDI mass spectrometry, including: a sample preparation process (S) which includes preparing a sample by mixing a specimen containing an organic compound to be analyzed with a predetermined matrix at a mixture ratio within a range from 1:5 to 1:5000 in molar ratio; a mass spectrometry process (S) which includes irradiating the prepared sample with a laser beam having a spot size equal to or smaller than 15 &#x3bc;m to generate ions originating from a component of the specimen in the sample, and performing a mass spectrometric analysis of the generated ions; and an analyzing process (S) which includes detecting, from a mass spectrum acquired in the mass spectrometry process, ions including product ions resulting from in-source decay, and estimating the structure of the organic compound to be analyzed based on information concerning the ions.

Method for structural analysis of organic compound

The present invention provides a method for specifically cleaving a C&#x3b1;-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a C&#x3b1;-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a C&#x3b1;-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry.

Method for specific cleavage of C alpha-C bond and side chain of protein and peptide, and method for determining amino acid sequence

The present invention provides a mass spectrometry method using a mixed matrix, capable of easily and efficiently improving the ionization efficiency in mass spectrometry for peptides having a wide range of degrees of hydrophobicity (from hydrophilic to hydrophobic). A MALDI mass spectrometry method for analyzing a sample using as a mixed matrix, 2,4,6-trihydroxyalkylphenone represented by the following general formula (I): wherein R represents an alkyl group having 3 to 12 carbon atoms, and a matrix (II) for mass spectrometry that is more hydrophilic than 2,4,6-trihydroxyalkylphenone represented by the formula (I) wherein R is an alkyl group having 3 carbon atoms.

Mass spectrometry method using mixed matrix

A thiol probe for a protein, which promotes ionization in proteomic analysis using mass spectrometry and can be used even for a protein that has a high degree of hydrophobicity and quickly turns over, and is represented by the following formula (I): In formula (I), Rrepresents a linker group, and Rrepresents a substituted ammonium group or a substituted amino group. A mass spectrometry method for a protein, includes the steps of obtaining a modified protein by reacting the thiol probe with a protein to be subjected to mass spectrometry and subjecting the modified protein to mass spectrometry.

Hydrophilic thiol probe

The present invention provides a method for mass spectrometry of phosphorylated peptides or sugar chains, which suppresses the desorption of an unstable site during ionization and achieves high sensitivity (i.e., detects molecular-related ions at a high ionic strength and at a relatively higher ionic strength than other ion species derived from the desorption of an unstable site). A method for mass spectrometry of phosphorylated peptides or sugar chains, the method comprising using, as a liquid matrix, an ionic liquid comprising a 3-aminoquinoline ion and a p-coumaric acid ion. The liquid matrix comprises 3-aminoquinoline and p-coumaric acid, for example, in a molar ratio of 5:1 to 20:1. Ammonium phosphate may be used as an additive.

Mass spectrometry method of phosphorylated peptides and sugar chains

The present invention provides a method for obtaining structural information conveniently and rapidly by generating a negative ion without adding an acidic substance to matrix, thereby improving sensitivity of measurement by a mass spectrometer, and by generating structural specific ions with high reproducibility, and a method for screening disease marker and a method for analyzing a sample containing a biomolecule. A method for mass spectrometry of a sugar chain comprising the steps of: preparing a sample containing a sugar chain; labeling the sugar chain with a labeling compound, to obtain a labeled sugar chain; and subjecting the labeled sugar chain to measurement of negative ions by using a MALDI mass spectrometer, thereby conducting analysis of the sugar chain. A method for screening disease marker comprising the steps of: (1) labeling the biomolecule X derived from a subject affected by a particular disease; subjecting the labeled biomolecule X' to measurement of negative ions by a MALDI mass spectrometer, (2) separately, labeling the biomolecule Y derived from a subject unaffected by the particular disease; subjecting the labeled biomolecule Y&#x2032; to measurement of negative ions by a MALDI mass spectrometer; and (3) comparing mass spectrum of the labeled biomolecule X&#x2032; obtained in (1) with mass spectrum of the labeled biomolecule Y&#x2032; obtained in (2) to find mass spectrum peaks which are mutually different, thereby ascertaining presence of structure involved in expression of the particular disease.

Method of negative-ion MALDI analysis of neutral labeled sugars and screening disease markers

A detection portion for detecting a first potential difference between hand and foot by applying a current across the hand and the foot of a subject through both of a hand electrode and a foot electrode and detecting a second potential difference between both hands or between both feet by applying a current across both hands or across both feet of the subject through any one of the hand electrodes and the foot electrodes, a first body composition calculation portion for calculating a first whole-body composition by using whole-body impedance based on a result of detection of the first potential difference, a correction portion for correcting two-limb impedance based on a result of detection of the second potential difference, and a second body composition calculation portion for calculating a second whole-body composition by using the corrected two-limb impedance are included.

Body composition monitor capable of accurately measuring whole-body composition and achieving facilitated manipulation

A health management guideline advising device includes input means for inputting personal information of a measuring subject; measurement means for measuring weight and body impedance of the measuring subject; estimation means for estimating a body composition distribution of the measuring subject from the inputted personal information and the measurement result by the measurement means; storage means for storing the measurement result by the measurement means and an estimation result by the estimation means with a measurement date and time, prediction means for predicting future weight fluctuation from the stored weight and the body composition distribution; and display means for displaying the predicted future weight fluctuation. Furthermore, target setting means for setting a weight reduction target and determination means for determining whether or not the weight reduction target can be achieved based on the prediction result by the prediction means are preferably arranged.

Health management guideline advising device

The present invention provides a liver preservation solution containing trehalose and dibutyryl-cAMP. The content of nitroglycerin in the preservation solution is preferably lower than 0.44 mM. In the liver preservation solution of the present invention, since the toxicity due to nitroglycerin, which is observed during liver preservation, has been improved, liver transplantation can be performed with a high engrafted rate.

Method for preserving liver in a solution comprising dibutyryl cAMP

A physical exercise assisting device includes a physical activity amount recorder recording a performed physical activity amount such that a physical exercise amount and a lifestyle activity amount can be recognized for a physical activity that has an intensity greater than or equal to a reference intensity; goal storage unit storing goals for the physical activity amount and the physical exercise amount to be performed in a predetermined unit period; and a display displaying goal achievement of the physical activity amount and the physical exercise amount based on the physical activity amount and the physical exercise amount recorded by the physical activity amount recorder and the goals stored in the goal storage unit.

Physical exercise assisting device

The present invention provides a simplified method for analyzing a structure of glycoprotein capable of conducting a peptide sequence analysis, a sugar chain sequence analysis, and an analysis of a sugar chain binding site accurately and rapidly using a mass spectrometer.

Method for analyzing structure of glycoprotein

A vibrator has massaging balls supported by a treating unit that may be shifted along a subject portion for treatment, guided by guiding members. The treating unit is supported by a supporting structure with respect to the guiding members. A position or orientation altering mechanism is provided for altering the position of the treatment unit in a direction orthogonal to the direction of its shifting or the orientation of the treating unit with respect to the guiding members. The supporting structure may include an engaging unit that engages the guiding members and an arm for supporting the engaging unit.

Vibrator, vibration unit, and vibrator control method

An X stage ( ) arranged on the front edge of a table ( ) is movable in the X-axis direction along a rail ( ). The X stage ( ) supports a light projecting device ( ) for illuminating a printed circuit board (PCB) and an imaging device ( ) for imaging an inspection area illuminated by the light projecting device ( ). An image obtained by the imaging device ( ) is displayed on a display device ( ). A Y stage ( ) is movably provided along a rail ( ) on a base ( ). A board supporting member ( ) for supporting the board (PCB) is pivotably attached to the Y stage ( ) at front portion thereof. An inspector judges whether or not soldering is acceptable while seeing the image displayed on the display device ( ). When it is judged that soldering is unacceptable, the inspector can correct an unacceptable soldered portion by drawing out the Y stage ( ) forward and obliquely raising the board supporting member ( ).

Visual inspection supporting apparatus and printed circuit board inspecting apparatus, and methods of soldering inspection and correction using the apparatuses

A method and device for supporting the repair of defective portions of a substrate when the defective portions have been discovered during an inspection process. A recorder records defective data associated with the defective portions. A data sorter sorts the defective data by grouping together defective data relating to a same type of defect. A data indicator highlights a defective location on the substrate where the defective portions were found in an order determined by defective data relating to the same type of defect or in an order determined by defective data relating to defects requiring the same type of repair. The data indicator also indicates the type of repair required and the tools to be used in the repair of the defective portions.

Growing community of inventors

Kyoto, Japan

Koichi Tanaka