Daiichi Sankyo Company, Limited

National Institute of Advanced Industrial Science and Technology

Kyowa Hakko Kirin Co., Limited

Koichi Nonaka

Kazuo Kobayashi

Yasunori Chiba

Kimihisa Ichikawa

Satoshi Baba

Yoshifumi Jigami

Kosuke Kuroda

Takeshi Suzuki

Yoshie Takahashi

Minako Ono

Kenji Masuda

Hiroki Tanemura

Masashi Tsuda

Rie Ito

The present invention provides an approach to enhancing the production of a foreign protein serving as a protein-based pharmaceutical product in host cells such as cultured cells derived from a mammal. The present invention provides transformed cells having a novel Hspa5 gene promoter, and a method for secreting and producing a foreign protein at high levels using the transformed host cells.

Promoter of Hspa5 gene

This invention provides a means for high-level secretory production of a protein, and, in particular, a protein having a complicated structure such as an antibody, in a host cell such as a yeast cell. This invention provides a method for high-level secretory production of a foreign protein with the use of a transformed host cell having one or more types of chaperone protein genes and via suppression of O sugar chain inherent to a host cell such as a yeast cell.

Method for high-level secretory production of protein

This invention provides a means for enabling high-level secretory production of proteins, in particular proteins having complicated structures such as antibodies, in host cells such as yeast cells. The invention also provides transformed yeast cells having the activated HAC1 gene and the RRBP1 gene and a method for enabling high-level secretory production of foreign proteins using such transformed host cells by inhibiting O-sugar chain formation indigenous to host cells such as yeast cells.

Growing community of inventors

Iwaki, Japan

Koichi Nonaka