Anaeropharma Science, Inc.

Azusapharma Sciences, Inc.

Koichi Koseki

Satoshi Kobayashi

Hitomi Shimizu

Yuko Shimatani

Takeshi Masaki

Koichiro Shioya

Tomio Matsumura

Masami Okabe

Kengo Inoue

Masao Tsukamoto

The object of the invention is to provide an anaerobic enterobacterium having a higher therapeutic effect on an anaerobic site such as a solid tumor tissue and an ischemic disease site. A bacterium of the genus, which is transformed with a plasmid co-expressing two types of heterologous polypeptides and comprising two types of secretory expression cassettes each sequentially comprising a promoter DNA functioning in the bacterium of the genus; a DNA encoding a secretory signal peptide; a DNA encoding a heterologous polypeptide; and a terminator DNA functioning in the bacterium of the genus, efficiently secretes the two types of heterologous peptides, i.e., two types of antibodies having anticancer effects, outside the bacterial cell.

Co-expression plasmid

An object is to provide a signal peptide that can secrete a heterologous polypeptide with high efficiency outside the bacterial cell in a bacterium of the genus, an expression cassette that can secrete a heterologous polypeptide with high efficiency outside the bacterial cell, a heterologous polypeptide expression vector, a bacterium of the genuscapable of secreting a heterologous polypeptide. Means for attaining the object is a bacterium of the genustransformed by a vector having an expression cassette sequentially comprising a promoter DNA functioning in a bacterium of the genus, a DNA encoding the secretory signal peptide, a DNA encoding a scFv antibody having an antitumor activity, and a terminator DNA functioning in the bacterium of the genus; and capable of secreting the scFv antibody with high efficiency outside the bacterial cell.

Heterologous polypeptide expression cassette

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Tokyo, Japan

Koichi Koseki