Daiichi Pure Chemicals Co., Ltd.

Arkray, Inc.

Koichi Hino

Mitsuhisa Manabe

Mitsuhiro Nakamura

Kazuo Nakanishi

Hiroshi Tamura

Takehiro Yamaguchi

Susumu Nishino

The present invention relates to a test piece having a simple structure, by which HDL cholesterol can be measured easily using a small amount of specimen. A reagent layer () is formed on a support (), and an enzyme reagent for measuring cholesterol, a first surfactant that makes a solubility of a high-density lipoprotein (HDL) higher than that of a lipoprotein other than the HDL, and a second surfactant that inhibits the lipoprotein other than the HDL from dissolving are contained in the reagent layer ().

Test piece for measuring high-density lipoprotein (HDL) cholesterol

Test piece for assaying high density lipoprotein (HDL) cholesterol

A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

Method for quantitatively determining LDL cholesterols

Method for quantitatively determining cholesterol

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Tokyo, Japan

Koichi Hino