Nissan Chemical Corporation

Kohei Suzuki

Yoshiomi Hiroi

Natsuki Abe

Hiroyuki Nakajima

Kazutaka Matoba

Keiichiro Otsuka

Satoshi Hamada

The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I): wherein Uand Ueach independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Rrepresents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Rrepresents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

Method for producing polymer used as base film for cell culture, and cell culture container

The invention relates to a cell culture container that inhibits adhesion of cells, a method for manufacturing the same and a method for producing cell aggregates using the same. The cell culture container has a coating film containing a copolymer having a recurring unit which contains a group represented by formula (a) and a recurring unit which contains a group represented by formula (b): wherein U, U, U, Uand Ueach independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Anrepresents an anion selected from the group consisting of a halide ion, an inorganic acid ion, a hydroxide ion and an isothiocyanate ion, on at least a part of a surface of the container, and the volume of the container is 20 μL or less.

Cell culture container having minute volume

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Funabashi, Japan

Kohei Suzuki