National Research Institute of Brewing

Fasmac Co., Ltd.

Kohei Shimamoto

Yusaku Wada

Ken Oda

Kazuhiro Iwashita

The present invention relates to a method of editing a filamentous fungal genome by direct introduction of a genome editing protein molecule or complex. The method includes three modes. In the first mode, a genome editing protein molecule or complex for a target gene is directly introduced into a cell of anfungus, to edit a gene in thefungal genome. In the second mode, a genome editing protein molecule or complex for a target region in a filamentous fungal genome, and a desired DNA fragment, are directly introduced into a filamentous fungal cell, to knock-in the DNA fragment to a desired target site in the filamentous fungal genome. In the third mode, genome editing protein molecules or complexes for plural target genes are directly introduced into a filamentous fungal cell, to carry out simultaneous editing of the plural genes in the filamentous fungal genome.

Growing community of inventors

Higashihiroshima, Japan

Kohei Shimamoto