Nara Institute of Science and Technology

Daiso Co., Ltd.

Aramis Biotechnologies Inc.

Ko Kato

Atsuhiko Shinmyo

Marc-Andre D'Aoust

Toshio Suzuki

Atsushi Nakagawa

Hideaki Idogaki

Kazuya Yoshida

Kiyotaka Ueda

Yoshikazu Tanaka

Pierre-Olivier Lavoie

Takaaki Kusumi

Renya Okawara

Michio Shibata

Yuhki Kubo

Ryutaro Aida

Tatsuya Nosho

Shingo Nagaya

Koji Akasaka

Pierre-Oliver Lavoie

Shigehiko Kanaya

Shotaro Yamasaki

Hideyuki Matsuura

Shinya Takenami

Toshihiro Yamura

An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbMT78 (SEQ ID NO:1); nbATL75 (SEQ ID NO:2); nbDJ46 (SEQ ID NO:3); nbCHP79 (SEQ ID NO:4); nbEN42 (SEQ ID NO:5); atHSP69 (SEQ ID NO:6); atGRP62 (SEQ ID NO:7); atPK65 (SEQ ID NO:8); atRP46 (SEQ ID NO:9); nb30S72 (SEQ ID NO:10); nbGT61 (SEQ ID NO:11); nbPV55 (SEQ ID NO:12); nbPPI43 (SEQ ID NO:13); nbPM64 (SEQ ID NO:14); and nbH2A86 (SEQ ID NO:15). Methods for using the isolated expression enhancer are also provided.

Plant expression enhancer

An isolated expression enhancer active in a plant, portion of a plant or plant cell, the expression enhancer is provided. The isolated expression enhancer may be selected from the group consisting of nbEPI42 (SEQ ID NO:1); nbSNS46 (SEQ ID NO:2); nbCSY65 (SEQ ID NO:3); nbHEL40 (SEQ ID NO:4); and nbSEP44 (SEQ ID NO:5). Methods for using the isolated expression enhancer are also provided.

Endogenous plant expression enhancer

An object of the present invention is to identify sequence features of the 5' UTR, which are involved in changes in the translational state of plants under environmental stresses, and to provide a recombinant DNA molecule, an expression vector, a transformant, and the like, having the above sequence features. The present invention provides a recombinant DNA molecule encoding mRNA containing the 5&#x2032; UTR (a) or (b) defined below, a vector obtained by ligating the recombinant DNA molecule to a site immediately downstream of the transcription initiation point of a promoter, and a transformant comprising the vector: (a) 5&#x2032; UTR in which a sequence of bases 1 to 7 and a sequence of bases 12 to 32 from the 5&#x2032; end are, respectively, a sequence of bases 1 to 7 and a sequence of bases 12 to 32 of SEQ ID NO: 4, 6, 20, 36, or 60; and (b) 5&#x2032; UTR in which one or more bases in the base sequence of the 5&#x2032; UTR of (a) are replaced, and which escapes translational repression induced by at least one environmental stress selected from the group consisting of heat stress and salt stress.

Recombinant DNA molecule encoding 5' UTR capable of preventing inhibition of translation under environmental stresses

The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived fromsp. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus, a EnHCH gene shown by base sequence of SEQ. ID. NO: 1, a gene encoding a protein having an amino acid sequence of SEQ. ID. NO: 2, andDH5&#x3b1; (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.

Chlorohydrin and hydroxycarboxylic ester asymmetric hydrolase gene

The present invention is to provide a gene having asymmetric hydrolase activity which is useful for synthesis of an optically active carboxylic acid, its antipode ester, and lactone, and a hydroxycarboxylic ester asymmetric hydrolase enzyme (EnHCH) derived from. DS-S-75 strain (FERM BP-5494) which is bacteria belonging to the genus, a EnHCH gene shown by base sequence of SEQ.ID.NO: 1, a gene encoding a protein having an amino acid sequence of SEQ.ID.NO: 2, andDH5&#x3b1; (pKK-EnHCH) deposited to International Patent Organism Depositary, National Institute of Advanced Industrial Science and Technology as a deposition No. FERM BP-08466.

A method useful for stable expression of an exogenous gene, introduced into a plant, is provided. By introducing an exogenous gene and 5&prime; upstream sequence of tobacco dehydogenase concurrently, stable expression of the exogenous gene introduced into a plant can be achieved.

DNA fragment for stable expression of an exogenous gene in a plant

A method for the stable expression of an introduced exogenous gene in a plant or plant cell is provided. Stable expression of an exogenous gene that was introduced was achieved by operably linking an upstream sequence of sea urchin arylsulfatase gene as an insulator.

Method of stable gene expression in a transgenic plant utilizing an insulator nucleotide sequence from the sea urchin arylsulfatase gene

Growing community of inventors

Ikoma, Japan

Ko Kato