Massachusetts Institute of Technology

Harvard College

Lumileds Lighting U.s., LLC

Philips Lumileds Lighting Company LLC

Other

Hewlett-packard Company

Klavs F Jensen

Moungi G Bawendi

Bashir Osama Dabbousi

Frederic Victor Mikulec

Armon R Sharei

Andrea Adamo

Robert Samuel Langer

Jeffrey N Miller

Ronald L Moon

Javier Rodriguez-Viejo

Jason Heine

Martin Arnold Schmidt

Patrick Louis Heider

Bradley Lether Pentelute

Mark David Simon

Timothy F Jamison

Aleksander Franz

J Christopher Love

Viktor A Adalsteinsson

Darrell J Irvine

Jie Chen

Ping Zhang

Kathleen M Vaeth

Ulrich Von Andrian

Pamela A Basto

Marc J Lajoie

Ryan Hartman

Allan Stuart Myerson

Samuel Benjamin Schaevitz

Dale Arlington Thomas, Iii

David R Snead

Jean-Christophe Monbaliu

James Robbins Abshire

Siddharth Jhunjhunwala

George M Whitesides

Marin Soljacic

John Dimitris Joannopoulos

Rajeev J Ram

Steven G Johnson

David C Duffy

Rebecca Jane Jackman

Peter H Fisher

Nathan E Stott

Harry Lee

Robert S Langer, Jr

Ivan Celanovic

Matthew W Bedore

Michael Ghebrebrhan

Peter Bermel

Alexander James Mijalis

Axel Guenther

Moungi E Bawendi

Nahyun Cho

Nopphon Weeranoppanant

Torsten Stelzer

Jacquin Clarence Niles

Connor Wilson Coley

Robert S Langer

Samara Firebaugh

Mohsen Behnam

Shin Yee Wong

Hemantkumar R Sahoo

Shirley Mao

Brian Yen

George Hartoularos

Sophia Liu

Leonel R Arana

Megan Heimann

Nikolay Zaborenko

Eve Marie Revalor

Tommi S Jaakkola

Gregory Lee Szeto

Walker Chan

Adrian Y X Yeng

Paolo Boccazzi

Regina Barzilay

Milad Abolhasani

Robert Langer

Robert Pilawa-Podgurski

Manish Jhunjhunwala

John R Naber

Jonathan P McMullen

Jacob Albrecht

Javier Rodriquez-Viejo

Angela Y Chen

Nicolas Szita

Andrea Zanzotto

Wengong Jin

Zhiyu Zhang

Xiaoyun Ding

Brian K H Yen

Jörg Lahann

Matthew W Losey

Xavier Rodriguez-Viejo

Gregory Szeto

Alexander P Clayton

Aaron A Bedermann

Kosisochukwu C Aroh

Christopher Marton

Mohammad Araghchini

The current subject matter includes methods, systems, articles, and techniques to deliver material to anucleate cells, such as red blood cells. Using a rapid deformation based microfluidic system, loading of red blood cells with macromolecules of different sizes has been shown. Although delivery to some mammalian cells, such as cancer cell lines and fibroblasts had been previously demonstrated using this technique, those designs were incompatible with RBCs that have dramatically different physical properties. Through the use of smaller constriction sizes, high speeds and different buffers successful delivery to red blood cells can be achieved. By enabling robust delivery to red blood cells in a simple, scalable manner, the current subject matter can be implemented in a diversity of applications that deliver material to study red blood cell diseases and/or use red blood cells as a therapeutic platform. Related apparatus, systems, techniques, and articles are also described.

Delivery of materials to anucleate cells

A method and device for preferentially delivering a compound such as an antigen to the cytosol of an immune cell. The method comprises passing a cell suspension comprising the target immune cell through a microfluidic device and contacting the suspension with the compound(s) or payload to be delivered.

Delivery of biomolecules to immune cells

Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described.

Gene editing through microfluidic delivery

Systems and processes for performing solid phase peptide synthesis are generally described. Solid phase peptide synthesis is a known process in which amino acid residues are added to peptides that have been immobilized on a solid support. In certain embodiments, the inventive systems and methods can be used to perform solid phase peptide synthesis quickly while maintaining high yields. Certain embodiments relate to processes and systems that may be used to heat, transport, and/or mix reagents in ways that reduce the amount of time required to perform solid phase peptide synthesis.

Solid phase peptide synthesis processes and associated systems

Isolating or identifying a cell based on a physical property of said cell can include providing a cell suspension; passing said suspension through a microfluidic channel that includes a constriction; passing the cell suspension through the constriction; and, contacting said cell suspension solution with a compound. The constriction can be sized to preferentially deform a relatively larger cell compared to a relatively smaller cell.

Selective delivery of material to cells

Systems and methods for synthesizing chemical products, including active pharmaceutical ingredients, are provided. Certain of the systems and methods described herein are capable of manufacturing multiple chemical products without the need to fluidically connect or disconnect unit operations when switching from one making chemical product to making another chemical product.

Systems and methods for synthesizing chemical products, including active pharmaceutical ingredients

The instant disclosure is related to fluidic distributors, fluidic systems, and associated methods and articles. Certain embodiments are related to fluidic distributors that comprise bays including fluidic connections with relative positions that substantially correspond to each other. In some embodiments, a fluidic distributor may comprise bays with electrical interfaces with relative positions that substantially correspond to each other.

Reconfigurable multi-step chemical synthesis system and related components and methods

Gene editing can be performed by introducing gene-editing components into a cell by mechanical cell disruption. Related apparatus, systems, techniques, and articles are also described. The methods and systems of the invention solve the problem of intracellular delivery of gene editing components and gene editing complexes to target cells. The results described herein indicate that delivery of gene editing components, e.g., protein, ribonucleic acid (RNA), and deoxyribonucleic acid (DNA), by mechanical disruption of cell membranes leads to successful gene editing. Because intracellular delivery of gene editing materials is a current challenge, the methods provide a robust mechanism to engineer target cells without the use of potentially harmful viral vectors or electric fields.

Aspects of the present disclosure relate to reconfigurable chemical synthesis systems and related components and methods. In one aspect, a fluidic system comprises a plurality of fluid outlets, a plurality of fluid inlets, a plurality of tensioners, and a plurality of flexible conduits associated with the plurality of tensioners, wherein at least one flexible conduit of the plurality of flexible conduits is configured to fluidically connect a fluid outlet of the plurality of fluid outlets and a fluid inlet of the plurality of fluid inlets. Another aspect relates to a method in which ends of a plurality of flexible conduits are physically moved along paths, one after the other, prior to flowing material through the flexible conduits, after which the ends of the flexible conduits are again physically moved.

Reconfigurable chemical synthesis systems and methods

Growing community of inventors

Lexington, MA, United States of America

Klavs F Jensen