Boehringer Mannheim Gmbh

Klaus Pollmann

Helmut Freitag

Anselm Rothe

Hans-Erich Wilk

Elmar Schmidt

Karin Munter

Rainer Fritsche

Johannes Winkle

Klaus Nohl

Henio Eikmeier

Subject matter of the invention is a new method of detecting a substance with hydrolase activity in a sample, characterized in that the sample is brought into contact with an indicator enzyme, which is covalently bound to an insoluble carrier material and can be rendered soluble by the hydrolase activity, in that the cleaved off indicator is separated and in that its enzymatic activity is determined and test means for its implementation.

Devices for determining hydrolase activity through its hydrolytic

The present invention provides a test carrier for the analytical determination of a component of a liquid sample, especially of a body fluid, with a carrier layer, which is partly covered by a liquid-absorbing layer which contains a sample application zone and a transport zone, and a reagent layer which is in contact with the liquid-absorbing layer, wherein the reagent layer in contact with the liquid-absorbing layer is so arranged that it does not overlap with the sample application zone and is full-facedly in contact with the liquid-absorbing layer.

Test carrier for the analytical determination of a component of a liquid

The present invention provides a test carrier for the analysis of a sample liquid with a test layer which contains a ligand fixed to to carrier particles, which ligand reacts with a component of the sample liquid, the test layer having a three-dimensional open structure which is so porous that the sample liquid with the component can penetrate into it, the carrier particles being arranged in the layer in such a manner that the ligand, upon pentration of the sample liquid, comes into contact with the component of the sample, wherein the carrier particles consist of an inorganic material, on the surface of which reactive organic groups are covalently bound as coupling groups, and the ligand is covalently bound to the coupling groups. The present invention also provides processes for the production of such a test carrier and a method for using it.

Test carrier for analysis of a liquid sample

Test carrier analysis system for the analytical determination of a component of a sample fluid, in particular of a body fluid. The system comprises test carriers (1) with a color formation layer (5) which is attached movably to the test carrier. The color formation layer is in the initial state of the test carrier not in fluid contact with a fluid reservoir layer (4) likewise attached to the test carrier, but can be pressed against the latter by external manipulation. The system also incorporates an evaluation apparatus with a pressure element (10a) for the pressing of the color formation layer (5) and a control unit (25) for controlling the apparatus functions. In order to improve the analytical determination as regards simplicity of handling and accuracy of the analysis, the reference measurement and the sample measurement are under the control of the control unit (25) carried out in turn on the color formation layer serving as reference surface by means of the same optical unit (1), after the color formation layer has been pressed against the fluid reservoir layer (4) by means of the pressure element (10a).

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Indianapolis, IN, United States of America

Klaus Pollmann