Roche Nimblegen, Inc.

Roche Diagnostics Operations, Inc.

Ventana Medical Systems, Inc.

Nigu Chemie Gmbh

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Klaus-Peter Stengele

Todd Richmond

Matthew Rodesch

Tom Albert

Roland D Green

Mark McCormick

Gary Barrett

Jochen Buehler

Markus Ott

Jochen Bühler

Heinrich Giegrich

The present disclosure relates to photoactivable protecting groups containing a diarylsulfide chromophore, a method for the synthesis thereof and their use as photoactivable protecting groups using maskless photolithography based array synthesis. 

Diarylsulfide backbone containing photolabile protecting groups

The present disclosure relates to photoactivable protecting groups containing a diarylsulfide chromophore, a method for the synthesis thereof and their use as photoactivable protecting groups using maskless photolithography based array synthesis.

The present description refers to photocleavable compounds which can be used as a photocleavable linker in order to link two biomolecules, such as oligonucleotides and peptides. The present description further refers to a method for the synthesis of said photocleavable compounds. 

Photocleavable linker molecules with diarylsulphide backbone for transient bioconjugate synthesis

An oligopeptide microarray and methods for the synthesis thereof are presented. Further presented is a microarray on a solid support comprising at least about 10,000 oligopeptide features per cmand preferably at least about 50,000 oligopeptide features per cm.

Methods for synthesis of an oligopeptide microarray

The present invention relates to a microarray comprising at least 50,000 oligopeptide features per cmwhere the oligopeptide features represent at least 50%, 60%, 70%, 80%, 90%, 95%, 99%, or 100% of the proteome of a virus or an organism. The present invention further relates to methods for the synthesis of such microarrays and methods of using microarrays comprising at least 50,000 oligopeptide features per cm. In an embodiment of the invention, the oligopeptide features represent proteins expressed in the same species, wherein the oligopeptide features are presented in a tiling pattern representing at least about 5,000 to-at least about 25,000 proteins expressed in a species. In some embodiments, the oligopeptide microarray features represent proteins expressed in the same species, wherein the microarray features are present in a tiling pattern that represents at least about 5,000 to at least about 50,000 expressed proteins.

Whole proteome tiling microarrays

The present invention is a microarray having a plurality of subarrays with a hydrophobic barrier that defines each subarray of the microarray, and a method for preparing such a microarray. The hydrophobic barrier is prepared using a microarray synthesis instrument, where NPPOC photoprotected and other hydrophobic group-bearing phosphoramidites are coupled to the microarray using light from a digital micromirror to direct formation of the hydrophobic barrier. The method utilizes hydrophobicity, a well-established property, of conventional phosphoramidite protecting groups for an entirely new application, the synthesis of hydrophobic barriers on microarrays.

Microarray with hydrophobic barriers

The present invention relates to novel nucleoside derivatives of general formula (I) wherein R=H, halogen, NO, CN, OCH, an alkyl, alkoxy or alkoxyalkyl residue having 1 to 4 C atoms, preferably a methyl, ethyl, propyl or butyl residue or an optionally substituted aryl residue or aliphatic acyl residue having 2 to 5 atoms, Rto R=H, NO, CN, OCH, a branched or unbranched alkyl, alkoxy or alkoxyalkyl residue having 1 to 5 C atoms or an optionally substituted aryl residue or an aliphatic acyl residue having 2 to 5 atoms, X is the group C&#x2550;O or C&#x2550;S, Y&#x2550;S, O, NR', C(R&#x2032;), wherein R&#x2032; is H, or a branched or unbranched alkyl residue having 1 to 5 C atoms or an optionally substituted aryl residue, Z=SO, OCO, OCS, SCS, and N is a nucleotide.

Photolabile protecting groups

The invention relates to a method for the specific photolytic deprotection of nucleoside derivatives that are immobilized on a substrate, especially for use in the production of DNA chips. Said method is characterized in that a gel or viscous liquid layer is applied on the nucleoside derivatives that are immobilized on a substrate. Said gel or viscous liquid contains one or more polymer compounds and at least one representative from the group comprising water, water/C -C alcohol mixtures and polar aprotic solvents. For initiating the deprotection, the nucleoside derivates are irradiated. This method favors a rapid, clean and complete removal of the photolabile protective groups from the nucleoside derivatives, which results in the required purity of the synthesized nucleotide or oligonucleotide sequences.

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Pleiskirchen, Germany

Klaus-Peter Stengele