Hoechst Aktiengesellschaft

Aventis Pharma Deutschland, Gmbh

Klaus-Peter Koller

Johannes Meiwes

Gunther J Riess

J&uuml;rgen Bongs

Wolfgang Kruse

Eugen Uhlmann

Wilfried Schwab

Werner Aretz

Rudiger Marquardt

Michael Dorschug

Holger Wallmeier

Gabriele Bierbaum

Hans Georg Sahl

The present invention relates to a process for delaying the deactivation of glutaryl amidase during enzyme catalysis. The enzyme catalyst is, after maximum conversion of the substrate, separated off by filtration and can be reused in a subsequent reaction batch. In each reaction cycle, the enzyme catalyst loses activity. The inactivation of the enzyme catalyst can be delayed by bringing it into contact with a thiol during the reaction.

Process for delaying the deactivation of glutaryl amidase during enzyme catalysis

The invention relates to the cell regulation factor TTO 20, DNA coding therefor, its preparation and use, antibodies binding to TTO 20 and the use of DNA coding for TTO 20 and an antibody binding to TTO 20 for use in a diagnostic kit for the detection of inflammatory disorders, in particular rheumatoid arthritis.

Cell regulation factor TTO 20

A process is provided for delaying the deactivation of glutaryl amidase during catalysis. The amidase is, after maximum conversion of a substrate, separated off by filtration and can be reused in a subsequent reaction batch. In each reaction cycle, the amidase loses activity. The inactivation of the amidase can be delayed by bringing it into contact with a thiol during the reaction. The thiol is preferably 2-mercaptoethanol or cysteine.

Process for delaying the deactivation of glutaryl amidase during catalysis with a thiol

The gene encoding glutarylacylase (GA) contained in the plasmid pCM 145 (DSM 6409) permits the expression of GA in E. coli in high yields.

Process for the preparation of glutarylacylase in large quantities

A process is described to specifically hydrolyze the amino acid chain of preproinsulin analogs to give the corresponding insulins. The hydrolysis is catalyzed by clostripain and, if necessary, carboxypeptidase B.

Clostripain catalyzed hydrolysis of preproinsulin analogs into

The present invention refers in particular to the structural gene sequence of the peptide antibiotic mersacidin. Sequencing revealed that premersacidin consists of an unusually long 48 amino acid leader sequence and a 20 amino acid propeptide part (Seq. ID No:1) which is modified during biosynthesis to the mature lantibiotic.

Recombinant mersacidin and a method for production

The tendamistat gene can be used for the construction of fused genes with which fusion proteins are expressed and excreted in Streptomycetes host cells. The tendamistat portion can be modified, in particular it can be C-terminal shortened. When a gene for a shortened proinsulin in which the insulin B chain is linked to the A chain only via Lys or Lys-Lys is coupled to the tendamistat gene, this gene construction is introduced into an expression vector, and the latter is used to transform a Streptomycetes host cell, there is expression and secretion of the corresponding fusion protein. The fusion protein can easily be cleaved to give insulin precursors because of correctly established disulfide bonds. Genetic structures which code for the signal sequence and about the first ten amino acids of tendamistat as well as a desired protein are expressed in streptomyces host cells with a high yield, and the fusion proteins are secreted into the medium.

Processes for the preparation of foreign proteins in streptomycetes

A tandem arrangement of two promoters which are each active in Streptomyces is provided. The tandem arrangement provides a considerable increase in protein expression.

Promoter arrangement for streptomycetes vectors

Cesium chloride is recovered from used density gradient solutions by extraction of lipophilic and coloring components using a lipophilic solvent, subsequent extraction with a polar protic organic solvent, adding perchlorate ions to the extraction residue, and converting the precipitated cesium perchlorate into cesium chloride.

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Bad Soden, Germany

Klaus-Peter Koller