National Research Council of Canada

Canadian Patents & Development Ltd.

Klaus Nielsen

John Walter Cherwonogrodzky

David R Bundle

Malcolm B Perry

J Robert Duncan

William C Hare

Bakhshish S Samagh

Alain M Bouillant

Peter F Wright

Gerda M Ruckerbauer

Permanent infection of a cell line such as a canine thymus cell line with a retrovirus such as equine infectious anemia virus and bovine Maedi-Visna-like virus is now possible. By culturing such an infected cell line under appropriate conditions, it is now possible to produce large quantities of viral antigens on a continuous basis. Such antigens are useful in for diagnostics and research.

Continuous production of bovine Maedi-Visna-like viral antigens in Cf2Th

A method is disclosed for discriminating between cattle vaccinated against and those infected with Brucella spp. The method involves immunoassay using a purified polysaccharide containing 4,6-dideoxy-4-acylamido-D-mannopyranosyl units obtained from B. abortus or from cross-reacting organisms, and results in improved differentiation between vaccinated and infected animals. Test kits are also disclosed for performing the assay and a process is disclosed for obtaining the O-chain polysaccharides in high purity and yield.

Immunoassays for discriminating between brucellosis infections and

Growing community of inventors

Richmond, Canada

Klaus Nielsen