Boehringer Mannheim Gmbh

Klaus Muehlegger

Herbert Von Der Eltz

Christian Birkner

Axel Heuser

Armin Reidel

Herbert Waldman

Hans-Joachim Hoeltke

Nucleotides labelled with an infrared dye and their use in nucleic acid detection

Process for the production of oligonucleotides of formula II, in which the exocyclic amino groups of the bases adenine, guanine, cytosine, 7-deazaadenine and 7-deazaguanine carry N-phenylacetyl groups, are used for oligonucleotide synthesis, wherein in a first step a starting nucleotide is bound to a solid carrier, subsequently the desired oligonucleotide is synthesized by stepwise coupling with appropriately activated further monomeric nucleotide building blocks of the general formula I with the above-mentioned meanings, if desired, trivalent phosphorus is oxidized to pentavalent phosphorus during and after the synthesis, the oligonucleotide is cleaved from the carrier and the 5' protecting groups are cleaved off. The phenylacetyl functional groups that protect exocyclic NH.sub.2 groups of the bases can be cleaved off in a mild way with penicillin amidohydrolase (EC 3.5.1.11).

Nucleosides and nucleoside derivatives containing enzymatically

Nucleosides, nucleotides and oligonucleotides containing enzymatically

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Polling, Germany

Klaus Muehlegger