Illumina Corporation

Illumina Cambridge Limited

Klaus Maisinger

John A Moon

Francisco Jose Garcia

Stephen Tanner

Tobias Mann

Anthony James Cox

Michael Lawrence Parkinson

Haifang H Ge

Kevin L Gunderson

Boyan Boyanov

Jonathan Mark Boutell

Roberto Rigatti

Matthew William Kellinger

Maria Candelaria Rogert Bacigalupo

Jingwei Bai

John M Beierle

Dale Buermann

Mark T Reed

Colin Lloyd Barnes

Mark R Pratt

Harold Philip Swerdlow

Jason Bryant

Eric Hans Vermaas

John Bridgham

Bojan Obradovic

Isabelle Rasolonjatovo

Gary Schroth

Saibal Banerjee

Kevin Benson

Sergey Etchin

Johnny Ho

Xavier Lee

Peter Lundberg

Chiara Rodighiero

Subra Sankar

Jonny Ho

Ning Sizto

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods. In particular, the present disclosure relates to a method for amplifying a nucleic acid on a substrate comprising at least one well, the method includes: depositing a plurality of first capture primer pairs and a plurality of second capture primer pairs in a layer at least partially covering the inner well surface, wherein the primer density of first capture primer pairs is higher than the primer density of second primer pairs; contacting a sample comprising a plurality of target polynucleotides with the substrate under conditions sufficient for a single target polynucleotide per well to hybridize with a capture primer of the second capture primer pair; and performing a kinetic exclusion assay (KEA) to produce a monoclonal population of amplicons from the single target polynucleotide.

Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid

Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

Creating a template of nucleic acid site locations on a flow cell

Analyzing image data from nucleic acid sequencing

Identifying nucleotides by determining phasing

The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods, including methods for modifying immobilized capture primers comprising: a) contacting a substrate comprising a plurality of immobilized capture primers with a plurality of template nucleic acids under conditions sufficient for hybridization to produce one or more immobilized template nucleic acids, and b) extending one or more immobilized capture primers to produce one or more immobilized extension products complementary to the one or more template nucleic acid.

System and methods for identifying nucleotides

Embodiments of the present disclosure relate to methods for capturing and amplifying target polynucleotides on a solid surface, in particular in a well in a microarray, wherein the microarray may comprise a) a substrate comprising at least one well, a surface surrounding the well and an inner well surface; b) a first layer covering the inner well surface and comprising at least one first capture primer pair; and c) a second layer covering the first layer and the surface surrounding the well.

Data processing system and methods

The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.

Systems and devices for sequence by synthesis analysis

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Essex, United Kingdom

Klaus Maisinger