Boehringer Mannheim Gmbh

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Klaus Kaluza

Guy Georges

Bruno Frey

Gudrun Schmitz-Agheguian

Herbert Von Der Eltz

Stefan Scheiblich

Matthias Koerner

Harald Sobek

Ulrike Reiff

Michael Jarsch

Christoph Kessler

Gunter Lang

Axel Schmidt

Johannes Auer

Manfred Schmidt

Dorothee Ambrosius

Gudrun Schmitz

Hans-Otto Beutler

Hans J Hoeltke

Stephan Gross

Wolfgang Von Der Saal

Thermolabile enzyme with uracil-DNA-glycosylase activity which is in particular characterized by a high degree of purity, short half-lives and a content of contaminating foreign activities of less than 2%, a process for its isolation as well as the use thereof to remove the base uracil from DNA and in particular from PCR products containing uracil. The enzyme is obtainable from gram-positive microorganisms such as e.g. Arthrobacter or Micrococcus.

Thermolabile uracil-DNA-glycosylas, process for its preparation and use for removing uracil from DNA

Oxygen-independent cholesterol-converting enzyme obtainable from Actinomycetes or Basidiomycetes which utilizes artificial electron acceptors as well as a method and reagent for the determination of (total) cholesterol using the enzyme.

Microbial cholesterol dehydrogenase, process for its production and use

The invention concerns a complex of cyclodextrin and an organic biocide, wherein the organic biocide has a maximum water-solubility of 0.15% (w/v) at a temperature of 25.degree. C., a process for the production of the complex as well as the use of the complex for preserving aqueous solutions. In addition the invention concerns a diagnostic test solution which contains at least one complex according to the invention and a preserved diagnostic test kit which comprises test reagents and at least one complex according to the invention.

Cyclodextrin-biocide complex

The new type II restriction endonuclease SexAI has the following recognition sequence and preferably cleaves at the cleavage site defined by the mark: ##STR1## It is preferably obtainable from microorganisms of the genus Streptomyces.

Type II restriction endonuclease SexAI

The invention concerns a new NAD.sup.+ -dependent D-malate dehydrogenase (decarboxylating, E.C. 1.1.1.83) from E. coli which consists of two subunits which each have a molecular weight of about 42 kD (SDS gel) and processes for the isolation of this enzyme from E. coli and for its stabilisation. The invention concerns in addition a method for the determination of D-malate with the D-malate dehydrogenase according to the present invention as well as a reagent for determining D-malate.

Enzymatic reagent for D-malate determination

The new type II restriction endonuclease Ssp4800I has the following recognition sequence: ##STR1## and preferably cleaves at the cleavage site defined by the arrows. It is preferably obtainable from microorganisms of the genus Streptomyces.

Type II restriction endonuclease Ssp4800I

Type II restriction endonuclease MamI

Type II restriction endonuclease McrI is disclosed. This endonuclease has recognition sequence: ##STR1## wherein R is G or A and Y is C or T, and the cleavage site indicated by the arrows. The endonuclease is preferably obtained from organism of genus Micrococcus. It can be used to recognize and cleave double stranded DNA sequence:

Type II restriction endonucleae MCRI

The type II restriction endonuclease RleAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Rhizobium. It can be used to recognize and cleave the double-stranded DNA sequence CCCACA(N).sub.12/9 and its complementary sequence.

Type II restriction endonuclease RleAI

The type III restriction endonuclease SgrAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from microorganisms of the genus Streptomyces. It can be used to recognize and cleave the double-stranded DNA sequence ##STR2## and its complementary sequence.

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Bad Heilbrunn, Germany

Klaus Kaluza