Other

Pharmacia Fine Chemicals Ab

Aktiebolaget Fermenta

Klaus H Mosbach

Per-Olof Larsson

Kurt G Nilsson

Kjell Nilsson

Lennart H Hansson

Mats-Olle Mansson

Kjell G Nilsson

Sten A Ohlson

Barbel G Hagerdal

Magnus J Glad

Particles which enclose cavities can be produced by adding a water-insoluble solid, liquid or gaseous cavity generating compound to an aqueous solution of matrix material. Subsequent to forming particles by dispersion in a water-insoluble dispersion medium, the matrix is rendered insoluble in water by cooling, by covalent cross-linking or by polymerization. The cavity generating compound is washed out, whereafter the particles can be used as ion exchangers in gel filtration processes, in hydrophobic chromatography or in affinity chromatography, optionally subsequent to derivatizing the particles. The particles can also be used to advantage as microcarriers in the cultivation of anchorage-dependent cells.

Macroporous particles for cell cultivation or chromatography

Particles which enclose cavities can be produced by adding a water-insoluble solid, liquid or gaseous cavity generating compound to an aqueous solution of a matrix material. Subsequent to forming particles by dispersion in a water-insoluble dispersion medium, the matrix is rendered insoluble in water by cooling, by covalent cross-linking or by polymerization. The cavity generating compound is washed out, whereafter the particles can be used as ion exchangers in gel filtration processes, in hydrophobic chromatography or in affinity chromatography, optionally subsequent to derivatizing the particles. The particles can also be used to advantage as microcarriers in the cultivation of anchorage dependent cells.

Preparation of catalysts for biochemical conversion reactions

The invention relates to a method of coupling organic ligands to a polymeric carrier. The organic ligand contains at least one primary or secondary amino group, at least one thiol group and/or at least one aromatic hydroxy group, and the polymeric carrier contains at least one hydroxy group. According to the invention the coupling is performed by forming a reactive derivative of the polymeric carrier by treatment with a sulfonyl halogenid, and reacting the formed reactive derivative with the organic ligand, which is thereby bonded directly to a carbon atom in the polymeric carrier. The polymeric carrier can, for example, be a possibly cross-linked polysaccharide, and the organic ligand is preferably a biological material such as a protein. The coupling product can, for example, be used for immunologic determinations, for affinity chromatography, etc. The coupling method can be performed under mild conditions not damaging sensitive organic ligands.

Method of covalently binding biologically active organic substances to

A chromatographic separation agent composed of a water unsoluble solid substance showing dihydroxyboryl groups. Said solid substance is structurally composed of a water unsoluble silica or silicate material as basic structure and, via a linkage to silicon atoms in the silica or silicate material, covalently bound dihydroxyboryl groups in acid, salt or complex form

Separation agent

Magnetic polymer particles

A method for activating immobilized living microorganisms applied to transformations of steroids, antibiotics and other compounds characterized by the addition of peptone, glucose or a mixture of peptone and glucose to the reaction mixture.

Growing community of inventors

Furulund, Sweden

Klaus H Mosbach