Instraction Gmbh

Dr. Gottschall Instruction Gesellschaft Fur Technische Chromatographie Mbh

Klawego Gmbh & Co. Kg

Dr. Gottschall Instruction Gesellschaft Fuer Technische Chromatografie Mbh

Dr Gottschall Instruction Gesellschaft Fuer Technische Chromatographie Mbh

Chiral Technologies Europe Sas

Klaus Gottschall

Martin Welter

Christian Meyer

Markus Weis

Markus Arendt

Lothar Ziser

Andres Kirschfeld

Lothar Britsch

Pilar Franco

Yasuto Morishita

Tong Zhang

Evelyn Gottschall

The present invention relates to composite materials useful for purifying proteins obtained from biological feedstocks. The composite materials of the invention comprise a porous support having an average pore size of 5 to 500 nm, said porous support being filled with a first polymer which is cross-linked, and a second polymer which is not crosslinked and is covalently bonded to the external surface of the cross-linked polymer-filled porous support.

Composite materials for bioseparations

Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rand the impurity compound being characterized by a hydrodynamic radius R, wherein R&#x3e;R, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that R&#x3c;Rand R&#x3e;R; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.

Use of a polymeric mesh for the purification of macromolecules

Sorbent comprising a solid support material, the surface of which comprises first residues comprising a binuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S, and second residues comprising a mononuclear heteroaromatic structure comprising besides carbon atoms at least one of the heteroatoms N, O, S.

Specific sorbent for binding proteins and peptides, and separation method using the same

Sorbent comprising a solid support material, the surface of which comprises a first residue comprising a pyridyl ring, whose hydrogen atoms may be substituted, and a second residue comprising a carboxyl group. The first and second residues may be attached to a polymer which coats a carrier, but which polymer is not covalently grafted to the carrier. The sorbents can be used to separate or to increase the concentration and/or purity of a protein or peptide from a mixture containing the same.

In a process, a polymer is crosslinked and then reversibly bound to a template that is dissolved or suspended in a solvent. The polymer thereby acquires a conformation adapted to the template such that an interaction enthalpy between the template and the polymer is increased by more than 0.1 kcal/mole. The template can be a chemical compound or a biological structure.

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Heddesheim, Germany

Klaus Gottschall