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Klaus Gersonde

Wilfried Schal

Murray Weiner

Hinrich Sick

For plotting an equilibrium curve representative of the relation between the oxygen saturation value of a hemoglobin sample, such as blood, and a changing oxygen partial pressure level in a gas, the sample is initially oxygenated by an oxygen-containing gas till it is completely saturated with oxygen, and thereafter by a controlled supply of deoxygenating gas to the volume of gas acting on the sample the oxygen partial pressure is decreased in a controlled manner. The oxygen saturation value is determined from the photometrically measured light absorption parameter of the sample in the measuring space.

Process and apparatus for plotting the oxygen equilibrium curves of

In accordance with a method of and an arrangement for producing lipid vesicles from biological membranes or lipid suspensions, a lipid suspension or lipid particles in a dispersion fluid are accommodated in a treatment container, ultrasonic treatment of the same is performed in condition of a substantially constant temperature, ultrasonic frequency and intensity for providing a desirable size or size distribution of the lipid vesicles are determined, and these parameters are maintained constant by measuring an actual value of frequency and intensity of an ultrasonic field of the reaction medium and adjusting a power and frequency of the ultrasonic treatment in dependence upon the measured actual value.

Method of and arrangement for producing lipid vesicles

Lipid vesicles, such as those containing inositol hexaphosphate useful for improving the oxygen-release of intact erythrocytes, may be rendered stable to long term storage at temperatures below the freezing point of the encapsulated solution, by rapid freezing at a temperature of from about -196.degree. C. to about -30.degree. C.

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Aachen, Germany

Klaus Gersonde